![Western Blot: KLF4 Antibody [NBP2-17070] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF4 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody..](http://images.novusbio.com/fullsize/KLF4-Antibody-Western-Blot-NBP2-17070-img0013.jpg "Western Blot: KLF4 Antibody [NBP2-17070] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF4 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody..")
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human KLF4. The exact sequence is proprietary. |
| Localization | Nucleus |
| Predicted Species | Porcine (100%), Bovine (100%), Rhesus Macaque (100%), Zebrafish (94%), Feline (100%), Sheep (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | KLF4 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
|
| Application Notes | ICC/IF Assay-dependent dilution |
|
| Theoretical MW | 55 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
|
| Publications |
|
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol, 1% BSA |
| Preservative | 0.025% Proclin 300 |
| Purity | Immunogen affinity purified |
![Western Blot SOX2 Antibody [Unconjugated]](https://images.novusbio.com/images2/SOX2_AF2018_Western_Blot_5161.jpg)
![Chromatin Immunoprecipitation (ChIP) SOX2 Antibody [Unconjugated]](https://images.novusbio.com/images2/SOX2_AF2018_Chromatin_Immunoprecipitation_11224.jpg)
![Simple Western SOX2 Antibody [Unconjugated]](https://images.novusbio.com/images2/SOX2_AF2018_Simple_Western_16676.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for KLF4 Antibody (NBP2-17070)Find related products by research area.
|
|
Breast cancer stem cells survive chemotherapy through S100A10-ANXA2-SPT6 interaction that epigenetically promotes OCT4-mediated stemness By Jamshed Arslan, Pharm D, PhDBreast cancer is the most common cancer among women that causes the greatest number of cancer-related deaths worldwide. After radiotherapy or cytotoxic chemotherapy like paclitax... Read full blog post. |
|
Deriving neural precursor cells from human induced pluripotent stem cells By Jennifer Sokolowski, MD, PhD.Human induced pluripotent stem cells (iPSCs) can be used to create models of human organ systems and are useful for a) ascertaining the mechanisms underlying pathological conditions... Read full blog post. |
|
Application Focus: New Methods for iPSC Differentiation, Inducing a Mammary Fate Discovery of the Key to PluripotencyInduced pluripotent stem cells (iPSCs) may be generated from a wide range of fully differentiated cells, and under optimal conditions may be prompted to differentiate into virtu... Read full blog post. |
|
KLF4 as a transcription factor in stem cell differentiation Kru¨ppel-like factors (KLFs) are evolutionarily conserved zinc finger transcription factors that play a role in cell differentiation, proliferation, and pluripotency. KLF4 has specifically been tied to many diverse cellular processes, including sel... Read full blog post. |
|
KLF4 opens the door for stem cell research KLF4 (Kruppel-like factor 4, Epithelial zinc finger protein EZF) is a zinc finger transcription factor thought to be involved in developmental differentiation and proliferation. It is considered a pluripotency reprogramming factor (PRF) due to its abi... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | KLF4 |
![Immunohistochemistry-Paraffin: KLF4 Antibody [NBP2-17070] - Mouse lung. KLF4 stained by KLF4 antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/KLF4-Antibody-Immunohistochemistry-Paraffin-NBP2-17070-img0016.jpg "Immunohistochemistry-Paraffin: KLF4 Antibody [NBP2-17070] - Mouse lung. KLF4 stained by KLF4 antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Flow Cytometry: KLF4 Antibody [NBP2-17070] - Human embryonic stem cells, staining with KLF4 antibody at 1:100 dilution (purple) or rabbit IgG (black).](http://images.novusbio.com/fullsize/KLF4-Antibody-Flow-Cytometry-NBP2-17070-img0005.jpg "Flow Cytometry: KLF4 Antibody [NBP2-17070] - Human embryonic stem cells, staining with KLF4 antibody at 1:100 dilution (purple) or rabbit IgG (black).")
![Western Blot: KLF4 Antibody [NBP2-17070] - Sample (20 ug of whole cell lysate) A: human ESC 10% SDS PAGE gel, diluted at 1:3000.](http://images.novusbio.com/fullsize/KLF4-Antibody-Western-Blot-NBP2-17070-img0001.jpg "Western Blot: KLF4 Antibody [NBP2-17070] - Sample (20 ug of whole cell lysate) A: human ESC 10% SDS PAGE gel, diluted at 1:3000.")
![Western Blot: KLF4 Antibody [NBP2-17070] - Sample (20 ug of whole cell lysate) A: mouse ESC 12% SDS PAGE gel, diluted at 1:3000.](http://images.novusbio.com/fullsize/KLF4-Antibody-Western-Blot-NBP2-17070-img0002.jpg "Western Blot: KLF4 Antibody [NBP2-17070] - Sample (20 ug of whole cell lysate) A: mouse ESC 12% SDS PAGE gel, diluted at 1:3000.")
![Western Blot: KLF4 Antibody [NBP2-17070] - Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE gel, diluted at 1:5000.](http://images.novusbio.com/fullsize/KLF4-Antibody-Western-Blot-NBP2-17070-img0004.jpg "Western Blot: KLF4 Antibody [NBP2-17070] - Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE gel, diluted at 1:5000.")
![Western Blot: KLF4 Antibody [NBP2-17070] - HCT116 whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF4 antibody diluted at 1:2000.](http://images.novusbio.com/fullsize/KLF4-Antibody-Western-Blot-NBP2-17070-img0008.jpg "Western Blot: KLF4 Antibody [NBP2-17070] - HCT116 whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF4 antibody diluted at 1:2000.")
![Western Blot: KLF4 Antibody [NBP2-17070] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with KLF4 antibody diluted at 1:5000.](http://images.novusbio.com/fullsize/KLF4-Antibody-Western-Blot-NBP2-17070-img0009.jpg "Western Blot: KLF4 Antibody [NBP2-17070] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with KLF4 antibody diluted at 1:5000.")
![Western Blot: KLF4 Antibody [NBP2-17070] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF4 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody](http://images.novusbio.com/fullsize/KLF4-Antibody-Western-Blot-NBP2-17070-img0011.jpg "Western Blot: KLF4 Antibody [NBP2-17070] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF4 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody")
![Immunohistochemistry-Paraffin: KLF4 Antibody [NBP2-17070] - Rat lung. KLF4 stained by KLF4 antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/KLF4-Antibody-Immunohistochemistry-Paraffin-NBP2-17070-img0014.jpg "Immunohistochemistry-Paraffin: KLF4 Antibody [NBP2-17070] - Rat lung. KLF4 stained by KLF4 antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Immunohistochemistry-Paraffin: KLF4 Antibody [NBP2-17070] - Rat kidney. KLF4 stained by KLF4 antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/KLF4-Antibody-Immunohistochemistry-Paraffin-NBP2-17070-img0015.jpg "Immunohistochemistry-Paraffin: KLF4 Antibody [NBP2-17070] - Rat kidney. KLF4 stained by KLF4 antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Immunoprecipitation: KLF4 Antibody [NBP2-17070] - Immunoprecipitation of KLF4 protein from HeLa whole cell extracts using 5 ug of KLF4 antibody. Western blot analysis was performed using KLF4 antibody EasyBlot anti-Rabbit IgG) was used as a secondary reagent.](http://images.novusbio.com/fullsize/KLF4-Antibody-Immunoprecipitation-NBP2-17070-img0007.jpg "Immunoprecipitation: KLF4 Antibody [NBP2-17070] - Immunoprecipitation of KLF4 protein from HeLa whole cell extracts using 5 ug of KLF4 antibody. Western blot analysis was performed using KLF4 antibody EasyBlot anti-Rabbit IgG) was used as a secondary reagent.")
![Chromatin Immunoprecipitation (ChIP) Oct-3/4 Antibody [Unconjugated]](https://images.novusbio.com/images2/Oct-3_4_AF1759_Chromatin_Immunoprecipitation_11218.jpg)
![Immunocytochemistry Oct-3/4 Antibody [Unconjugated]](https://images.novusbio.com/images2/Oct-3_4_AF1759_Immunocytochemistry_7093.jpg)
![Simple Western Oct-3/4 Antibody [Unconjugated]](https://images.novusbio.com/images2/Oct3_4_AF1759_Simple_Western_20183.jpg)
![Chromatin Immunoprecipitation (ChIP) Nanog Antibody [Unconjugated]](https://images.novusbio.com/images2/Nanog_AF1997_Chromatin_Immunoprecipitation_11222.jpg)
![Immunocytochemistry Nanog Antibody [Unconjugated]](https://images.novusbio.com/images2/Nanog_AF1997_Immunocytochemistry_6478.jpg)
![Immunocytochemistry Nanog Antibody [Unconjugated]](https://images.novusbio.com/images2/Nanog_AF1997_Immunocytochemistry_6636.jpg)
![Immunocytochemistry LIN-28A Antibody [Unconjugated]](https://images.novusbio.com/images2/LIN-28A_AF3757_Immunocytochemistry_6502.jpg)
![Immunocytochemistry LIN-28A Antibody [Unconjugated]](https://images.novusbio.com/images2/LIN-28A_AF3757_Immunocytochemistry_12362.jpg)
![Western Blot LIN-28A Antibody [Unconjugated]](https://images.novusbio.com/images2/LIN-28A_AF3757_Western_Blot_9318.jpg)
![LIF [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "LIF [Unconjugated]")
![Simple Western KLF5 Antibody [Unconjugated]](https://images.novusbio.com/images2/KLF5_AF3758_Simple_Western_17008.jpg)
![Knockout Validated KLF5 Antibody [Unconjugated]](https://images.novusbio.com/images2/KLF5_AF3758_Knockout_Validated_22484.jpg)
![Immunocytochemistry KLF5 Antibody [Unconjugated]](https://images.novusbio.com/images2/KLF5_AF3758_Immunocytochemistry_12361.jpg)
![N/A PSG1 [HRP]](https://images.novusbio.com/images2/DATA_PSG1_DPSG10_ELISA_797.jpg)
![N/A PSG1 [HRP]](https://images.novusbio.com/images2/PSG-1_DPSG10_ELISA_164.jpg)
