KLF4 Antibodies
We offer KLF4 Antibodies for use in common research applications: ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot. Each KLF4 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our KLF4 Antibodies can be used in a variety of model species: Bovine, Human, Mouse, Primate, Rat. Use the list below to choose the KLF4 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our KLF4 polyclonal antibodies and browse our KLF4 monoclonal antibody catalog.
Alternate Names for KLF4 Antibodies
anti-KLF4 antibody, anti-Epithelial zinc finger protein EZF antibody, anti-EZFendothelial Kruppel-like zinc finger protein antibody, anti-GKLFKrueppel-like factor 4 antibody, anti-Gut-enriched krueppel-like factor antibody, anti-Kruppel-like factor 4 (gut) antibody
12 Results for "klf4" in Primary Antibodies
![Immunohistochemistry: KLF4 Antibody [NBP2-24749] - DAB IHC staining. Representative 3,3-diaminobenzidine immunohistochemical-stained images showing protein expression of induced-pluripotent stem cell marker KLF4 (J-L, brown) in normal colon (J), low-grade (K) and high-grade (L) colon adenocarcinoma tissue samples. Nuclei were counter-stained with hematoxylin (A-O, blue). Original magnification: 400x. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0221963) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/KLF4-Antibody-Immunohistochemistry-NBP2-24749-img0006.jpg "Immunohistochemistry: KLF4 Antibody [NBP2-24749] - DAB IHC staining. Representative 3,3-diaminobenzidine immunohistochemical-stained images showing protein expression of induced-pluripotent stem cell marker KLF4 (J-L, brown) in normal colon (J), low-grade (K) and high-grade (L) colon adenocarcinoma tissue samples. Nuclei were counter-stained with hematoxylin (A-O, blue). Original magnification: 400x. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0221963) licensed under a CC-BY license.")
![Western Blot: KLF4 Antibody [NBP2-24749] - Total protein from Human PC3, Hek293 and Mouse Neuro2A cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-KLF4 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/KLF4-Antibody-Western-Blot-NBP2-24749-img0005.jpg)
![Immunohistochemistry: KLF4 Antibody [NBP2-24749] - IF IHC staining. Representative immunofluorescence immunohistochemical-stained images showing protein expression of induced-pluripotent stem cell markers OCT4 (A-C, green), KLF4 (A-C, red) in normal colon (A), low-grade (B) and high-grade (C) colon adenocarcinoma tissue samples. Cell nuclei were counter-stained with 4', 6'-diamidino-2-phenylindole (A-C, blue). Original magnification: 400x. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0221963) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/KLF4-Antibody-Immunohistochemistry-NBP2-24749-img0007.jpg)
![Immunohistochemistry-Paraffin: KLF4 Antibody [NBP2-24749] - Representative immunofluorescence immunohistochemical-stained sections of colon adenocarcinoma metastasis to the liver showing the expression of KLF4 [red] on the cells within the tumor nests (TNs) [thin arrows] and those within the peritumoral stroma [arrowheads]. OCT4 [ green] was expressed on the KLF4+ [green, arrowheads] cells within the peritumoral stroma, and also cells within the peritumoral stroma that expressed KLF4 [ red, thick arrows] not expressing OCT4. c-Myc was expressed by cells within the TNs [green, arrows] and those within the peritumoral stroma [arrowheads]. The c-Myc+ population within the peritumoral stroma also expressed KLF4 [red]. Cell nuclei were counterstained with DAPI [blue]. Scale bars: 20 um. Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fsurg.2017.00076/full), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/KLF4-Antibody-Immunohistochemistry-Paraffin-NBP2-24749-img0008.jpg)
![Flow Cytometry: KLF4 Antibody [NBP2-24749] - Intracellular analysis using KLF4 antibody. Balb/c mouse splenocytes were probed using 1 ug of KLF4 antibody (red) and 1 ug of isotype control was used for this test, and an anti-rabbit IgG PE conjugated secondary .](http://images.novusbio.com/fullsize/KLF4-Antibody-Flow-Cytometry-NBP2-24749-img0003.jpg)
![Western Blot: KLF4 Antibody [NBP2-24749] - Lysate from human 293 cells in the (1) absence and (2) presence of immunizing peptide, (3) mouse NIH 3T3, and (4) RAW cells probed with KLF4 antibody at 1 ug/ml (293) and 2 ug/ml (NIH 3T3 and RAW). Goat anti-rabbit IgG HRP secondary and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/KLF4-Antibody-Western-Blot-NBP2-24749-img0001.jpg)
![Immunohistochemistry-Paraffin: KLF4 Antibody [NBP2-24749] - Breast cancer tissues embedded in paraffin. Negative control is no primary antibody. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/KLF4-Antibody-Immunohistochemistry-Paraffin-NBP2-24749-img0004.jpg)
![Western Blot: KLF4 Antibody [NBP1-83940] - Analysis in human cell lines PC-3 and U2OS using Anti-KLF4 antibody. Corresponding KLF4 RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/KLF4-Antibody-Western-Blot-NBP1-83940-img0014.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: KLF4 Antibody [NBP1-83940] - Analysis in human cell lines PC-3 and U2OS using Anti-KLF4 antibody. Corresponding KLF4 RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: KLF4 Antibody [NBP1-83940] - Staining in human skin and pancreas tissues. Corresponding KLF4 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/KLF4-Antibody-Immunohistochemistry-Paraffin-NBP1-83940-img0021.jpg)
![Western Blot: KLF4 Antibody [NBP1-83940] - ZNF217 and ALKBH5 regulate NANOG and KLF4 expression via modulation of m6A levels. MCF-7 subclones were exposed to 20% or 1% O2 for 48 h and immunoblot assays were performed. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/article/11743/text/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/KLF4-Antibody-Western-Blot-NBP1-83940-img0025.jpg)
![Immunocytochemistry/Immunofluorescence: KLF4 Antibody [NBP1-83940] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/KLF4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83940-img0009.jpg)
![Western Blot: KLF4 Antibody [NBP1-83940] - Human breast cancer cell MCF-7 was treated with carboplatin, alone or in combination with Stat3 inhibitor, for 72 hours, and the expression of Klf4 was detected by Western blot. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/KLF4-Antibody-Western-Blot-NBP1-83940-img0022.jpg)
![Western Blot: KLF4 Antibody [NBP1-83940] - Analysis in control (vector only transfected HEK293T lysate) and KLF4 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/KLF4-Antibody-Western-Blot-NBP1-83940-img0023.jpg)
![Western Blot: KLF4 Antibody [NBP1-83940] - MDA-MB-231 subclones transfected with NTC or HIF-1 alpha shRNA vector were treated with vehicle (V) or paclitaxel (P) and immunoblot assay was performed. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/KLF4-Antibody-Western-Blot-NBP1-83940-img0024.jpg)
![Immunohistochemistry-Paraffin: KLF4 Antibody [NBP1-83940] - Staining of human colon shows moderate to strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/KLF4-Antibody-Immunohistochemistry-Paraffin-NBP1-83940-img0017.jpg)
![Immunohistochemistry-Paraffin: KLF4 Antibody [NBP1-83940] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/KLF4-Antibody-Immunohistochemistry-Paraffin-NBP1-83940-img0018.jpg)
![Immunohistochemistry-Paraffin: KLF4 Antibody [NBP1-83940] - Staining of human skin shows moderate to strong nuclear positivity in keratinocytes.](http://images.novusbio.com/fullsize/KLF4-Antibody-Immunohistochemistry-Paraffin-NBP1-83940-img0019.jpg)
![Immunohistochemistry-Paraffin: KLF4 Antibody [NBP1-83940] - Staining of human testis shows moderate to strong nuclear positivity in a subset of cells in seminiferous ducts.](http://images.novusbio.com/fullsize/KLF4-Antibody-Immunohistochemistry-Paraffin-NBP1-83940-img0020.jpg)
![Western Blot: KLF4 Antibody [NBP2-17070] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF4 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody..](http://images.novusbio.com/fullsize/KLF4-Antibody-Western-Blot-NBP2-17070-img0013.jpg "Western Blot: KLF4 Antibody [NBP2-17070] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF4 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody..")
![Immunohistochemistry-Paraffin: KLF4 Antibody [NBP2-17070] - Mouse lung. KLF4 stained by KLF4 antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/KLF4-Antibody-Immunohistochemistry-Paraffin-NBP2-17070-img0016.jpg)
![Flow Cytometry: KLF4 Antibody [NBP2-17070] - Human embryonic stem cells, staining with KLF4 antibody at 1:100 dilution (purple) or rabbit IgG (black).](http://images.novusbio.com/fullsize/KLF4-Antibody-Flow-Cytometry-NBP2-17070-img0005.jpg)
![Western Blot: KLF4 Antibody [NBP2-17070] - Sample (20 ug of whole cell lysate) A: human ESC 10% SDS PAGE gel, diluted at 1:3000.](http://images.novusbio.com/fullsize/KLF4-Antibody-Western-Blot-NBP2-17070-img0001.jpg)
![Western Blot: KLF4 Antibody [NBP2-17070] - Sample (20 ug of whole cell lysate) A: mouse ESC 12% SDS PAGE gel, diluted at 1:3000.](http://images.novusbio.com/fullsize/KLF4-Antibody-Western-Blot-NBP2-17070-img0002.jpg)
![Western Blot: KLF4 Antibody [NBP2-17070] - Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE gel, diluted at 1:5000.](http://images.novusbio.com/fullsize/KLF4-Antibody-Western-Blot-NBP2-17070-img0004.jpg)
![Western Blot: KLF4 Antibody [NBP2-17070] - HCT116 whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF4 antibody diluted at 1:2000.](http://images.novusbio.com/fullsize/KLF4-Antibody-Western-Blot-NBP2-17070-img0008.jpg)
![Western Blot: KLF4 Antibody [NBP2-17070] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with KLF4 antibody diluted at 1:5000.](http://images.novusbio.com/fullsize/KLF4-Antibody-Western-Blot-NBP2-17070-img0009.jpg)
![Western Blot: KLF4 Antibody [NBP2-17070] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF4 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody](http://images.novusbio.com/fullsize/KLF4-Antibody-Western-Blot-NBP2-17070-img0011.jpg)
![Immunohistochemistry-Paraffin: KLF4 Antibody [NBP2-17070] - Rat lung. KLF4 stained by KLF4 antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/KLF4-Antibody-Immunohistochemistry-Paraffin-NBP2-17070-img0014.jpg)
![Immunohistochemistry-Paraffin: KLF4 Antibody [NBP2-17070] - Rat kidney. KLF4 stained by KLF4 antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/KLF4-Antibody-Immunohistochemistry-Paraffin-NBP2-17070-img0015.jpg)
![Immunoprecipitation: KLF4 Antibody [NBP2-17070] - Immunoprecipitation of KLF4 protein from HeLa whole cell extracts using 5 ug of KLF4 antibody. Western blot analysis was performed using KLF4 antibody EasyBlot anti-Rabbit IgG) was used as a secondary reagent.](http://images.novusbio.com/fullsize/KLF4-Antibody-Immunoprecipitation-NBP2-17070-img0007.jpg)
![Western Blot: KLF4 Antibody (7B7U1) [NBP3-15469] - Analysis of extracts of Mouse lung, using KLF4 Rabbit mAb (NBP3-15469) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/KLF4-Antibody-7B7U1-Western-Blot-NBP3-15469-img0007.jpg "Western Blot: KLF4 Antibody (7B7U1) [NBP3-15469] - Analysis of extracts of Mouse lung, using KLF4 Rabbit mAb (NBP3-15469) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunocytochemistry/Immunofluorescence: KLF4 Antibody (7B7U1) [NBP3-15469] - Immunofluorescence analysis of U-2 OS cells using KLF4 Rabbit mAb (NBP3-15469) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/KLF4-Antibody-7B7U1-Immunocytochemistry-Immunofluorescence-NBP3-15469-img0005.jpg)
![Immunohistochemistry-Paraffin: KLF4 Antibody (7B7U1) [NBP3-15469] - Mouse spinal cord using KLF4 Rabbit mAb (NBP3-15469) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/KLF4-Antibody-7B7U1-Immunohistochemistry-Paraffin-NBP3-15469-img0003.jpg)
![Western Blot: KLF4 Antibody (7B7U1) [NBP3-15469] - Analysis of extracts of various cell lines, using KLF4 Rabbit mAb (NBP3-15469) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/KLF4-Antibody-7B7U1-Western-Blot-NBP3-15469-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: KLF4 Antibody (7B7U1) [NBP3-15469] - Immunofluorescence analysis of C6 cells using KLF4 Rabbit mAb (NBP3-15469) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/KLF4-Antibody-7B7U1-Immunocytochemistry-Immunofluorescence-NBP3-15469-img0004.jpg)
![Immunohistochemistry-Paraffin: KLF4 Antibody (7B7U1) [NBP3-15469] - Rat brain using KLF4 Rabbit mAb (NBP3-15469) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/KLF4-Antibody-7B7U1-Immunohistochemistry-Paraffin-NBP3-15469-img0001.jpg)
![Immunohistochemistry-Paraffin: KLF4 Antibody (7B7U1) [NBP3-15469] - Human esophageal using KLF4 Rabbit mAb (NBP3-15469) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/KLF4-Antibody-7B7U1-Immunohistochemistry-Paraffin-NBP3-15469-img0002.jpg)
![Immunoprecipitation: KLF4 Antibody (7B7U1) [NBP3-15469] - Analysis of 300ug extracts of HeLa cells using 3ug KLF4 antibody (NBP3-15469). Western blot was performed from the immunoprecipitate using KLF4 antibody (NBP3-15469) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/KLF4-Antibody-7B7U1-Immunoprecipitation-NBP3-15469-img0008.jpg)
![Western Blot: KLF4 Antibody (CL5785) [NBP2-61427] - Analysis in human cell line U-2197.](http://images.novusbio.com/fullsize/KLF4-Antibody-CL5785-Western-Blot-NBP2-61427-img0008.jpg "Western Blot: KLF4 Antibody (CL5785) [NBP2-61427] - Analysis in human cell line U-2197.")
![Immunocytochemistry/Immunofluorescence: KLF4 Antibody (CL5785) [NBP2-61427] - Staining of U-2197cells using the Anti-KLF4 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/KLF4-Antibody-CL5785-Immunocytochemistry-Immunofluorescence-NBP2-61427-img0002.jpg)
![Immunohistochemistry-Paraffin: KLF4 Antibody (CL5785) [NBP2-61427] - Staining of human skeletal muscle shows absence of positivity in striated muscle fibers as expected (negative control).](http://images.novusbio.com/fullsize/KLF4-Antibody-CL5785-Immunohistochemistry-Paraffin-NBP2-61427-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: KLF4 Antibody (CL5785) [NBP2-61427] - Staining of PC-3 cells using the Anti-KLF4 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/KLF4-Antibody-CL5785-Immunocytochemistry-Immunofluorescence-NBP2-61427-img0001.jpg)
![Immunohistochemistry-Paraffin: KLF4 Antibody (CL5785) [NBP2-61427] - Staining of human stomach shows moderate to strong nuclear immunoreactivity in glandular cells.](http://images.novusbio.com/fullsize/KLF4-Antibody-CL5785-Immunohistochemistry-Paraffin-NBP2-61427-img0003.jpg)
![Immunohistochemistry-Paraffin: KLF4 Antibody (CL5785) [NBP2-61427] - Staining of human skin shows strong nuclear positivity in epithelial cells.](http://images.novusbio.com/fullsize/KLF4-Antibody-CL5785-Immunohistochemistry-Paraffin-NBP2-61427-img0004.jpg)
![Immunohistochemistry-Paraffin: KLF4 Antibody (CL5785) [NBP2-61427] - Staining of human endometrium shows nuclear immunoreactivity in glandular and stromal cells.](http://images.novusbio.com/fullsize/KLF4-Antibody-CL5785-Immunohistochemistry-Paraffin-NBP2-61427-img0005.jpg)
![Western Blot: KLF4 Antibody (CL5782) [NBP2-61426] - Analysis in human cell line U-2197.](http://images.novusbio.com/fullsize/KLF4-Antibody-CL5782-Western-Blot-NBP2-61426-img0007.jpg "Western Blot: KLF4 Antibody (CL5782) [NBP2-61426] - Analysis in human cell line U-2197.")
![Immunocytochemistry/Immunofluorescence: KLF4 Antibody (CL5782) [NBP2-61426] - Staining of U-2197cells using the Anti-KLF4 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/KLF4-Antibody-CL5782-Immunocytochemistry-Immunofluorescence-NBP2-61426-img0002.jpg)
![Immunohistochemistry-Paraffin: KLF4 Antibody (CL5782) [NBP2-61426] - Staining of human skeletal muscle shows absence of positivity in striated muscle fibers as expected (negative control).](http://images.novusbio.com/fullsize/KLF4-Antibody-CL5782-Immunohistochemistry-Paraffin-NBP2-61426-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: KLF4 Antibody (CL5782) [NBP2-61426] - Staining of PC-3 cells using the Anti-KLF4 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/KLF4-Antibody-CL5782-Immunocytochemistry-Immunofluorescence-NBP2-61426-img0001.jpg)
![Immunohistochemistry-Paraffin: KLF4 Antibody (CL5782) [NBP2-61426] - Staining of human rectum shows strong nuclear immunoreactivity in glandular cells.](http://images.novusbio.com/fullsize/KLF4-Antibody-CL5782-Immunohistochemistry-Paraffin-NBP2-61426-img0003.jpg)
![Immunohistochemistry-Paraffin: KLF4 Antibody (CL5782) [NBP2-61426] - Staining of human placenta shows nuclear positivity in the trophoblast.](http://images.novusbio.com/fullsize/KLF4-Antibody-CL5782-Immunohistochemistry-Paraffin-NBP2-61426-img0004.jpg)
![Immunohistochemistry-Paraffin: KLF4 Antibody (CL5782) [NBP2-61426] - Staining of human prostate shows nuclear immunoreactivity in glandular cells.](http://images.novusbio.com/fullsize/KLF4-Antibody-CL5782-Immunohistochemistry-Paraffin-NBP2-61426-img0005.jpg)
![Immunoprecipitation: KLF4 Antibody (3A1) [NBP3-26180] - Immunoprecipitating KLF4 in Hela whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26180 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26180 (2ug) + Hela whole cell lysate (500ug). Lane 3: Hela whole cell lysate (10ug).](http://images.novusbio.com/fullsize/nbp3-26180_rabbit-klf4-mab-3a1-262202413221520.jpg "Immunoprecipitation: KLF4 Antibody (3A1) [NBP3-26180] - Immunoprecipitating KLF4 in Hela whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26180 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26180 (2ug) + Hela whole cell lysate (500ug). Lane 3: Hela whole cell lysate (10ug).")
![Immunohistochemistry: KLF4 Antibody (3A1) [NBP3-26180] - Image of KLF4 Antibody (3A1) diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26180_rabbit-klf4-mab-3a1-262202414471565.jpg)
![Immunohistochemistry: KLF4 Antibody (3A1) [NBP3-26180] - Image of KLF4 Antibody (3A1) diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26180_rabbit-klf4-mab-3a1-27220248274912.jpg)
![Western Blot: KLF4 Antibody (3A1) [NBP3-26180] - Positive Western Blot detected in: Hela whole cell lysate, 293T whole cell lysate, PC-3 whole cell lysate, MCF-7 whole cell lysate, A549 whole cell lysate, U87 whole cell lysate, Brain tissue. All lanes: KLF4 Antibody at 1: 1500 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 55, 52, 46, 13, 7 kDa Observed band size: 55 kDa](http://images.novusbio.com/fullsize/nbp3-26180_rabbit-klf4-mab-3a1-28220241141363.jpg)
![Immunocytochemistry/Immunofluorescence: KLF4 Antibody (3A1) [NBP3-26180] - Staining of Hela Cells with KLF4 Antibody (3A1) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26180_rabbit-klf4-mab-3a1-2822024131222.jpg)
![Western Blot: KLF4 Antibody (4E6) [NBP1-21047] - Analysis of KLF4 in HeLa cells. The cell was stained with ATGA0129 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).](http://images.novusbio.com/fullsize/KLF4-Antibody-4E6-Western-Blot-NBP1-21047-img0003.jpg "Western Blot: KLF4 Antibody (4E6) [NBP1-21047] - Analysis of KLF4 in HeLa cells. The cell was stained with ATGA0129 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).")
![Flow Cytometry: KLF4 Antibody (4E6) [NBP1-21047] - Analysis of KLF4 in A549 cells. The cell was stained at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).](http://images.novusbio.com/fullsize/KLF4-Antibody-4E6-Flow-Cytometry-NBP1-21047-img0002.jpg)
![Western Blot: KLF4 Antibody (4E6) [NBP1-21047] - The recombinant protein (20ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KLF4 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: Recombinant human KLF4 protein](http://images.novusbio.com/fullsize/KLF4-Antibody-4E6-Western-Blot-NBP1-21047-img0001.jpg)
![Knockout Validated: KLF4 Antibody [NBP3-03529] - Analysis of extracts from normal (control) and KLF4 knockout (KO) HeLa cells, using KLF4 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/KLF4-Antibody-Knockout-Validated-NBP3-03529-img0001.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: KLF4 Antibody [NBP3-03529] - Analysis of extracts from normal (control) and KLF4 knockout (KO) HeLa cells, using KLF4 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.")
![Western Blot: KLF4 Antibody (S03-2I8) [NBP3-19883] - Western blot detection of KLF4 in Hela, CHO-K1 using NBP3-19883 (1:1000 diluted)](http://images.novusbio.com/fullsize/KLF4-Antibody-S03-2I8-Western-Blot-NBP3-19883-img0001.jpg "Western Blot: KLF4 Antibody (S03-2I8) [NBP3-19883] - Western blot detection of KLF4 in Hela, CHO-K1 using NBP3-19883 (1:1000 diluted)")