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Integrin beta 5 Antibody [Unconjugated]

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Integrin beta 5 was detected in immersion fixed MG-63 human osteosarcoma cell line using Sheep Anti-Human Integrin beta 5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3824) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; NL010) and counterstained with DAPI(blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Integrin beta 5 was detected in immersion fixed MG-63 human osteosarcoma cell line using Sheep Anti-Human Integrin beta 5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3824) at 10 µg/mL for 3 hours at ...read more
Simple Western lane view shows lysates of MG‑63 human osteosarcoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Integrin  beta 5 at approximately 129 kDa (as indicated) using 50 µg/mL of ...read more
Western blot shows lysates of HT‑29 human colon adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Integrin  beta 5 Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, Flow, CyTOF-ready, ICC/IF
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAF3824
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF3824-SP
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Integrin beta 5 Antibody [Unconjugated] Summary

Immunogen
Chinese hamster ovary cell line CHO cell-derived recombinant human Integrin beta 5
Gly24-Asn719
Accession # P18084
Specificity
Detects human Integrin  beta 5 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse (rm) Integrin beta 5 is observed, and less than 5% cross-reactivity with recombinant human (rh) Integrin  beta 1, rhIntegrin  beta 3, rhIntegrin  beta 4, rhIntegrin  beta 6 and rmIntegrin  beta 7 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
ITGB5
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Simple Western 50 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF3824 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Integrin beta 5 Antibody [Unconjugated]

  • FLJ26658
  • Integrin beta 5
  • integrin beta-5
  • integrin, beta 5
  • ITGB5

Background

Integrin  beta 5 is a 100‑110 kDa type I transmembrane glycoprotein that forms a non-disulfide-linked heterodimer with Integrin  alpha V. Integrin  alpha V beta 5 is a receptor for vitronectin that is expressed on a wide variety of cell types including keratinocytes, fibroblasts, adhesive monocytes, and select endothelium. The 696 amino acid residue Integrin  beta 5 extracellular region contains a vWF-A domain and a cysteine-rich tandem repeat region. Human Integrin  beta 5 shares 91% and 89% amino acid sequence homology with mouse and canine Integrin  beta 5, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Integrin beta 5 Antibody (AF3824)(5)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: ICC, Western Blot.


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ICC
(2)
Western Blot
(1)
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(2)
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(1)
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Bioinformatics

Gene Symbol ITGB5
Uniprot