Reactivity | HuSpecies Glossary |
Applications | PAGE, Bioactivity |
Format | Carrier-Free |
Concentration | LYOPH |
Description | A single non-glycosylated polypeptide chain containing 176 amino acids corresponding to Indian Hedgehog/Ihh Source: E. coli Uniprot ID: Q14623 Amino Acid Sequence: IIGPGRVVGS RRRPPRKLVP LAYKQFSPNV PEKTLGASGR YEGKIARSSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDRLNSLAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRNKYGLL ARLAVEAGFD WVYYESKAHV HCSVKSEHSA AAKTGG |
Details of Functionality | Indian Hedgehog Protein is fully biologically active when compared to standard. The ED50 as determined by its ability to induce alkaline phosphatase production by C3H10T1/2(CCL-226) cells is 3.0-10 ug/ml. |
Source | E. coli |
Protein/Peptide Type | Recombinant Protein |
Gene | IHH |
Purity | >95%, by SDS-PAGE and HPLC |
Endotoxin Note | Less than 1 EU/ug of Indian Hedgehog/Ihh as determined by LAL method. |
Dilutions |
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Theoretical MW | 19.8 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Storage | Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Buffer | Lyophilized from a 0.2 um filtered concentrated solution in 1 x PBS, 0.02% Tween-20, pH 7.0. |
Preservative | No Preservative |
Concentration | LYOPH |
Purity | >95%, by SDS-PAGE and HPLC |
Reconstitution Instructions | Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C. |
Research Areas for Indian Hedgehog/Ihh Recombinant Protein (NBP2-35012)Find related products by research area.
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