ILT6/CD85e/LILRA3 Overexpression Lysate

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

LILRA3, also known as Leukocyte immunoglobulin-like receptor subfamily A member 3, has 3 isoforms, a 439 amino acid isoform that is 47 kDa, a 375 amino acid isoform that is 40 kDa, and a 456 amino acid isoform that is 49; detected in B-cells, and at lower levels in natural killer (NK) cells, and in peripheral blood monocytes and lung; lacks a transmembrane region and is presumed to be a soluble receptor and may act as soluble receptor for class I MHC antigens. Studies on this protein have shown relationship with the following diseases and disorders: multiple sclerosis, rheumatoid arthritis, arthritis, ascariasis, trichuriasis, multiple sclerosis, rheumatism, interferon, and hepatitis c. This protein has been shown to interact with EWSR1 and USP46 proteins in osteoclast differentiation, defense response, and signal transduction pathways.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Array NBL1-12525

• ILT6/CD85e/LILRA3 Antibodies
• ILT6/CD85e/LILRA3 Antibody Pair
• ILT6/CD85e/LILRA3 ELISA Kits
• ILT6/CD85e/LILRA3 Lysate
• ILT6/CD85e/LILRA3 Proteins and Enzyme