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Ikaros/IKZF1 Antibody

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Immunohistochemistry-Paraffin: Ikaros/IKZF1 Antibody [NBP1-51990] - (3.8ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: Ikaros/IKZF1 Antibody [NBP1-51990] - (3.8ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, Bv, CaSpecies Glossary
Applications ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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Ikaros/IKZF1 Antibody Summary

Immunogen
Peptide with sequence C-DTPYDSSASYEKEN, from the internal region of the protein sequence according to NP_006051.1; NP_001278766.1;NP_001278767.1;NP_001207696.1;NP_001207697.1; NP_001278768.1; NP_001207699.1; NP_001207700.1.
Epitope
C-DTPYDSSASYEKEN
Specificity
This antibody is expected to recognise all isoforms NP_001278766.1;NP_001278767.1;NP_001207696.1;NP_001207697.1; NP_001278768.1; NP_001207699.1; NP_001207700.1
Predicted Species
Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IKZF1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 2.5 - 3.8 ug/ml
  • Immunohistochemistry-Paraffin 2.5 - 3.8 ug/ml
  • Peptide ELISA Detection limit 1:1000
Application Notes
WB: Preliminary testing showed a band at approx. 60kDa in Human Lymph node and Peripheral Blood Monocyte lysate, after 1ug/ml antibody staining (calculated MW of 57.5kDa according to NP_006051.1). Primary incubation 1 hour at room temperature. IHC-P: Human tonsil shows textured staining in some nuclei of the lymphoid tissue.
Publications
Read Publication using NBP1-51990.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Ikaros/IKZF1 Antibody

  • DNA-binding protein Ikaros
  • hIk-1
  • IK1
  • IK1LyF-1
  • Ikaros (zinc finger protein)
  • IKAROS family zinc finger 1 (Ikaros)
  • Ikaros family zinc finger protein 1
  • Ikaros
  • IKAROSLymphoid transcription factor LyF-1
  • IKZF1
  • LYF1
  • LyF-1
  • LYF1PRO0758
  • PRO0758
  • zinc finger protein, subfamily 1A, 1 (Ikaros)
  • ZNFN1A1
  • ZNFN1A1CLL-associated antigen KW-6

Background

IKZF1 is a transcription regulator of hematopoietic cell differentiation. Binds gamma-satellite DNA. Binds with higher affinity to gamma satellite A. Plays a role in the development of lymphocytes, B- and T-cells. Binds and activates the enhancer (delta-A element) of the CD3-delta gene. Repressor of the TDT (terminal deoxynucleotidyltransferase) gene during thymocyte differentiation. Regulates transcription through association with both HDAC-dependent and HDAC-independent complexes. Targets the 2 chromatin-remodeling complexes, NuRD and BAF (SWI/SNF), in a single complex (PYR complex), to the beta-globin locus in adult erythrocytes. Increases normal apoptosis in adult erythroid cells. Confers early temporal competence to retinal progenitor cells (RPCs) (By similarity).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol IKZF1
Entrez
Uniprot