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Goat anti-Feline IgM Heavy Chain Secondary Antibody

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Flow Cytometry: Goat anti-Feline IgM Heavy Chain Secondary Antibody [NB7258] - The cat cells were washed and Incubated with the IgM antibody. It was used to detect Bcells. This image was submitted via customer review. ...read more

Product Details

Summary
Reactivity FeSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Goat anti-Feline IgM Heavy Chain Secondary Antibody Summary

Immunogen
Cat IgM
Specificity
By immunoelectrophoresis and ELISA this reacts specifically with cat IgM. This may cross react with IgM from other species.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IGHM
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • ELISA 1:1000 - 1:30000
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:2000
  • Immunohistochemistry 1:200 - 1:2000
  • Immunohistochemistry-Paraffin 1:200 - 1:2000
  • Western Blot 1:1000- 1:30000
Application Notes
ELISA 1:1,000 - 1:30,000; for coating plates 1:100 - 1:500
Publications
Read Publications using NB7258.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Phosphate Buffered Saline (PBS)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Goat anti-Feline IgM Heavy Chain Secondary Antibody

  • Constant region of heavy chain of IgM
  • Ig Mu Chain C Region BOT
  • Ig Mu Chain C Region GAL
  • Ig Mu Chain C Region OU
  • immunoglobulin heavy constant mu
  • Immunoglobulin mu chain

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IgM Heavy Chain Antibody (NB7258)(7)

We have publications tested in 2 applications: ELISA, ICC/IF.


Filter By Application
ELISA
(2)
ICC/IF
(1)
All Applications
Filter By Species
All Species
Showing Publications 1 - 7 of 7.
Publications using NB7258 Applications Species
Park ES, Fujita O, Kimura M, et al. Diagnostic system for the detection of severe fever with thrombocytopenia syndrome virus RNA from suspected infected animals PloS one 2021-01-28 [PMID: 33507990] (ELISA) ELISA
Park ES, Shimojima M, Nagata N et al. Severe Fever with Thrombocytopenia Syndrome Phlebovirus causes lethal viral hemorrhagic fever in cats Sci Rep 2019-08-21 [PMID: 31427690] (ICC/IF, ELISA) ICC/IF, ELISA
Zulpo DL, Headley SA, Biazzono L et al. Oocyst shedding in cats vaccinated by the nasal and rectal routes with crude rhoptry proteins of Toxoplasma gondii. Exp Parasitol. [PMID: 22542988]

Details:
Citation using the HRP form of this antibody.
Romen F, Pawlita M, Sehr P et al. Antibodies against Gag are diagnostic markers for feline foamy virus infections while Env and Bet reactivity is undetectable in a substantial fraction of infected cats. Virology . [PMID: 16297422]

Details:
Citation using the HRP form of this antibody.
Burkhard MJ, Hoover EA. IgG from acutely infected cats blocks mucosal feline immunodeficiency virus infection. Vet Immunol Immunopathol. [PMID: 15910995]

Details:
Citation using the HRP form of this antibody.
Fujino Y, Ma Z, Satoh H et al. Characterization of a newly established nonproducer lymphoma cell line for feline leukemia virus. Vet Immunol Immunopathol 2004-12-01 [PMID: 15541796]
Omori M, Pu R, Tanabe T et al. Cellular immune responses to feline immunodeficiency virus (FIV) induced by dual-subtype FIV vaccine. Vaccine 2004-12-01 [PMID: 15530685]

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol IGHM