![Flow Cytometry: Rat anti-Mouse IgM Secondary Antibody (II/41) [Biotin] [NBP1-43546] - Staining of C57BL/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) APC and 0.06 ug of Rat IgG2a kappa Isotype Control Biotin (left) or 0.06 ug of Anti-Mouse IgM Biotin (right) followed by Streptavidin PE . Total viable cells were used for analysis.](http://images.novusbio.com/fullsize/Rat-anti-Mouse-IgM-Secondary-Antibody-II-41-Biotin-Flow-Cytometry-NBP1-43546-img0001.jpg "Flow Cytometry: Rat anti-Mouse IgM Secondary Antibody (II/41) [Biotin] [NBP1-43546] - Staining of C57BL/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) APC and 0.06 ug of Rat IgG2a kappa Isotype Control Biotin (left) or 0.06 ug of Anti-Mouse IgM Biotin (right) followed by Streptavidin PE . Total viable cells were used for analysis.")
| Reactivity | MuSpecies Glossary |
| Applications | Flow |
| Clone | II/41 |
| Clonality | Monoclonal |
| Host | Rat |
| Conjugate | Biotin |
| Concentration | 0.5 mg/ml |
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Allophycocyanin | NBP1-42995 | |||
| Biotin | NBP1-43546-0.1mg | |||
| FITC | NBP1-43947-0.05mg | |||
| PE | NBP1-43763-0.2mg | |||
| PE | NBP1-43763-0.1mg | |||
| PE | NBP1-43763-0.05mg | |||
| PE/Cy5 | NBP1-43068-0.1mg | |||
| PE/Cy7 | NBP1-42940-0.1mg | |||
| PE/Cy7 | NBP1-42940-0.05mg | |||
| Unconjugated | NBP1-43303-0.5mg | |||
| Immunogen | The immunogen for this antibody was IgM. |
| Isotype | IgG2a Kappa |
| Clonality | Monoclonal |
| Host | Rat |
| Gene | IGHM |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | The II/41 antibody has been tested by flow cytometric analysis of mouse spleen cell suspension. This can be used at less than or equal to 1 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test. |
| Readout System |
| Storage | Store at 4C in the dark. |
| Buffer | PBS (pH 7.2) with 0.1% gelatin |
| Preservative | 0.09% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
![IgM Antibody (II/41) [Biotin]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "IgM Antibody (II/41) [Biotin]")
Secondary Antibodies |
Isotype Controls |
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | IGHM |
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