![Western Blot: IgG2a Antibody (RM219) [Unconjugated] [NBP2-62008] - Western blot of nonreduced(-) and reduced(+) mouse IgG2a, using 0.5ug/mL of NBP2-62008B. This antibody reacts to nonreduced IgG2a (~150 kDa). Image using the Biotin form of this antibody.](http://images.novusbio.com/fullsize/Rabbit-anti-Mouse-IgG2a-Secondary-Antibody-RM219-Unconjugated-Western-Blot-NBP2-62008-img0003.jpg "Western Blot: IgG2a Antibody (RM219) [Unconjugated] [NBP2-62008] - Western blot of nonreduced(-) and reduced(+) mouse IgG2a, using 0.5ug/mL of NBP2-62008B. This antibody reacts to nonreduced IgG2a (~150 kDa). Image using the Biotin form of this antibody.")
| Reactivity | MuSpecies Glossary |
| Applications | WB, ELISA |
| Clone | RM219 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Mouse IgG2a |
| Specificity | This antibody reacts to the Fc region of mouse IgG2a. No cross reactivity with mouse IgG1, IgG2b, IgG2c, IgG3, IgM, IgA, IgE, human IgG, or rat IgG. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Publications |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | 50% Glycerol/PBS, 1% BSA |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
| Publication using NBP2-62008 | Applications | Species |
|---|---|---|
| Jang JH, Kim DH, Lim JM et al. Breast cancer cell-derived soluble CD44 promotes tumor progression by triggering macrophage IL-1 beta production Cancer Res. 2020-01-22 [PMID: 31969374] |
Secondary Antibodies |
Isotype Controls |
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![ELISA: IgG2a Antibody (RM219) [Unconjugated] [NBP2-62008] - A titer ELISA of mouse IgG2a. The plate was coated with different amount of mouse IgG2a. A serial dilution of NBP2-62008B was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody. Image using the Biotin form of this antibody.](http://images.novusbio.com/fullsize/Rabbit-anti-Mouse-IgG2a-Secondary-Antibody-RM219-Unconjugated-ELISA-NBP2-62008-img0001.jpg "ELISA: IgG2a Antibody (RM219) [Unconjugated] [NBP2-62008] - A titer ELISA of mouse IgG2a. The plate was coated with different amount of mouse IgG2a. A serial dilution of NBP2-62008B was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody. Image using the Biotin form of this antibody.")
![ELISA: IgG2a Antibody (RM219) [Unconjugated] [NBP2-62008] - ELISA of mouse immunoglobulins shows NBP2-62008B reacts to the Fc region of mouse IgG2a; no cross reactivity with IgG1, IgG2b, IgG2c, IgG3, IgM, IgA, IgE, human IgG, or rat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10ng/mL of NBP2-62008B was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody. Image using the Biotin form of this antibody.](http://images.novusbio.com/fullsize/Rabbit-anti-Mouse-IgG2a-Secondary-Antibody-RM219-Unconjugated-ELISA-NBP2-62008-img0002.jpg "ELISA: IgG2a Antibody (RM219) [Unconjugated] [NBP2-62008] - ELISA of mouse immunoglobulins shows NBP2-62008B reacts to the Fc region of mouse IgG2a; no cross reactivity with IgG1, IgG2b, IgG2c, IgG3, IgM, IgA, IgE, human IgG, or rat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10ng/mL of NBP2-62008B was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody. Image using the Biotin form of this antibody.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
