![Western Blot: IgG2a Antibody (RM219) [Biotin] [NBP2-62008B] - Western blot of nonreduced(-) and reduced(+) mouse IgG2a, using 0.5 ug/mL of NBP2-62008B. This antibody reacts to nonreduced IgG2a (~150 kDa).](http://images.novusbio.com/fullsize/Rabbit-anti-Mouse-IgG2a-Secondary-Antibody-RM219-Biotin-Western-Blot-NBP2-62008B-img0001.jpg "Western Blot: IgG2a Antibody (RM219) [Biotin] [NBP2-62008B] - Western blot of nonreduced(-) and reduced(+) mouse IgG2a, using 0.5 ug/mL of NBP2-62008B. This antibody reacts to nonreduced IgG2a (~150 kDa).")
| Reactivity | MuSpecies Glossary |
| Applications | WB, ELISA |
| Clone | RM219 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Biotin |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Mouse IgG2a |
| Specificity | This antibody reacts to the Fc region of mouse IgG2a. No cross reactivity with mouse IgG1, IgG2b, IgG2c, IgG3, IgM, IgA, IgE, human IgG, or rat IgG. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This primary antibody can also be used as a secondary antibody. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS, 50% Glycerol, 1% BSA |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
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![ELISA: IgG2a Antibody (RM219) [Biotin] [NBP2-62008B] - ELISA of mouse immunoglobulins shows NBP2-62008B reacts to the Fc region of mouse IgG2a; no cross reactivity with IgG1, IgG2b, IgG2c, IgG3, IgM, IgA, IgE, human IgG, or rat IgG. The plate was coated with different immunoglobulins. NBP2-62008B was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.](http://images.novusbio.com/fullsize/Rabbit-anti-Mouse-IgG2a-Secondary-Antibody-RM219-Biotin-ELISA-NBP2-62008B-img0002.jpg "ELISA: IgG2a Antibody (RM219) [Biotin] [NBP2-62008B] - ELISA of mouse immunoglobulins shows NBP2-62008B reacts to the Fc region of mouse IgG2a; no cross reactivity with IgG1, IgG2b, IgG2c, IgG3, IgM, IgA, IgE, human IgG, or rat IgG. The plate was coated with different immunoglobulins. NBP2-62008B was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.")
![ELISA: IgG2a Antibody (RM219) [Biotin] [NBP2-62008B] - A titer ELISA of mouse IgG2a. The plate was coated with different amount of mouse IgG2a. A serial dilution of NBP2-62008B was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.](http://images.novusbio.com/fullsize/Rabbit-anti-Mouse-IgG2a-Secondary-Antibody-RM219-Biotin-ELISA-NBP2-62008B-img0005.jpg "ELISA: IgG2a Antibody (RM219) [Biotin] [NBP2-62008B] - A titer ELISA of mouse IgG2a. The plate was coated with different amount of mouse IgG2a. A serial dilution of NBP2-62008B was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.")
