Home » IFN-alpha H2/IFNA14 » IFN-alpha H2 Lysates » IFN-alpha H2/IFNA14 Overexpression Lysate
Novus Biologicals products are now on bio-techne.com

IFN-alpha H2/IFNA14 Overexpression Lysate

 Submit a Review

Images

 
(
Enlarge)
Western Blot: IFNA14 Overexpression Lysate (Adult Normal) [NBP2-08142] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for IFNA14.
Western Blot: IFNA14 Overexpression Lysate (Adult Normal) [NBP2-08142] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for IFNA14.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

IFN-alpha H2/IFNA14 Overexpression Lysate Summary

Description

IFN-alpha H2/IFNA14 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC212411

Accession#: NM_002172

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Gene
IFNA14

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (cat# NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
21.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for IFN-alpha H2/IFNA14 Overexpression Lysate

  • IFNA14
  • IFNalpha H2
  • IFN-alpha H2
  • IFN-alpha-14
  • IFN-alphaH
  • interferon alpha-14
  • Interferon alpha-H
  • Interferon lambda-2-H
  • interferon, alpha 14
  • LeIF H
  • LEIF2H
  • MGC125756
  • MGC125757

Background

Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

IFN-alpha 1
Antibody
NBP2-75930
Western Blot IFN-alpha 1 Antibody
Immunocytochemistry/ Immunofluorescence IFN-alpha 1 Antibody
Immunocytochemistry/ Immunofluorescence IFN-alpha 1 Antibody
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
IFN-alpha 13
Antibody (3F9)
H00003447-M03
Western Blot IFN-alpha 13 Antibody (3F9)
Sandwich ELISA IFN-alpha 13 Antibody (3F9)
Species: Hu
Applications: ELISA, S-ELISA, WB
IFN-alpha I/IFNA17
Antibody
H00003451-D01P
Immunocytochemistry/ Immunofluorescence IFN-alpha I/IFNA17 Antibody
Western Blot IFN-alpha I/IFNA17 Antibody
Western Blot IFN-alpha I/IFNA17 Antibody
Species: Hu, Rt
Applications: ICC/IF, WB
CBP/KAT3A Antibody
(257003) [Unconjugate ...
MAB2676
Western Blot CBP/KAT3A Antibody (257003) [Unconjugated]
Immunocytochemistry CBP/KAT3A Antibody (257003) [Unconjugated]
(2 Publications)
Species: Hu, Mu, Rt
Applications: ICC, WB
IFN-beta
[Unconjugated]
8499-IF
Binding Activity IFN-beta [Unconjugated]
(47 Publications)
Species: Hu
Applications: BA
IRF7 Antibody
(SC0617)
NBP2-67634
Immunocytochemistry/ Immunofluorescence IRF7 Antibody (SC0617)
Immunocytochemistry/ Immunofluorescence IRF7 Antibody (SC0617)
Immunohistochemistry-Paraffin IRF7 Antibody (SC0617)
(1 Publication)
Species: Hu, Mu, Rt, Ze
Applications: Flow, ICC/IF, IHC, IHC-P, IP, WB
IFN-alpha
C/IFN-alpha 10 [Uncon
11120-1
IFN-alpha C/IFN-alpha 10 [Unconjugated]
(1 Publication)
Species: Hu
IFN-alpha
[Unconjugated]
11200-2
IFN-alpha [Unconjugated]
(31 Publications)
Species: Hu
PAG1 Antibody
(504004) [Unconjugate
MAB5285
Simple Western PAG1 Antibody (504004) [Unconjugated]
Western Blot PAG1 Antibody (504004) [Unconjugated]
(1 Publication)
Species: Hu
Applications: Simple Western, WB
IFN-alpha J1/IFNA7
Antibody
NBP1-79586
Western Blot IFN-alpha J1/IFNA7 Antibody
Species: Hu
Applications: WB
Cytokeratin 17
Antibody
NBP2-38536
Immunocytochemistry/ Immunofluorescence Cytokeratin 17 Antibody
Immunocytochemistry/ Immunofluorescence Cytokeratin 17 Antibody
Immunocytochemistry/ Immunofluorescence Cytokeratin 17 Antibody
(1 Review)
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
eIF4E [p Ser209]
Antibody
NB100-81898
Western Blot eIF4E [p Ser209] Antibody
Immunocytochemistry/ Immunofluorescence eIF4E [p Ser209] Antibody
Immunohistochemistry-Paraffin eIF4E [p Ser209] Antibody
(1 Publication)
Species: Hu, Po
Applications: ICC/IF, IHC, IHC-P, WB
Coagulation Factor
XIV/Protein C Antibod ...
NBP1-86509
Immunohistochemistry-Paraffin Coagulation Factor XIV/Protein C Antibody
Immunohistochemistry-Paraffin Coagulation Factor XIV/Protein C Antibody
Immunohistochemistry-Paraffin Coagulation Factor XIV/Protein C Antibody
(1 Publication)
Species: Hu
Applications: IHC, IHC-P
Podocalyxin Like
Antibody [Unconjugate ...
AF1556
Immunohistochemistry Podocalyxin Like Antibody [Unconjugated]
Western Blot Podocalyxin Like Antibody [Unconjugated]
Immunocytochemistry/ Immunofluorescence Podocalyxin Like Antibody [Unconjugated]
(3 Reviews)
(97 Publications)
Species: Mu
Applications: CyTOF-ready, Flow, IHC, WB
Opsin 1 (Long
Wave) Antibody
NBP3-09392
Western Blot Opsin 1 (Long Wave) Antibody
Species: Hu, Mu
Applications: WB
MuRF1/TRIM63
Antibody [Unconjugate
AF5366
Western Blot MuRF1/TRIM63 Antibody [Unconjugated]
Simple Western MuRF1/TRIM63 Antibody [Unconjugated]
Western Blot MuRF1/TRIM63 Antibody [Unconjugated]
(26 Publications)
Species: Hu, Mu, Rt
Applications: Simple Western, WB
CCL5/RANTES [HRP]
DRN00B
N/A CCL5/RANTES [HRP]
N/A CCL5/RANTES [HRP]
N/A CCL5/RANTES [HRP]
(100 Publications)
Species: Hu
Applications: ELISA
IFN-alpha I/IFNA17
[Unconjugated]
11150-1
IFN-alpha I/IFNA17 [Unconjugated]
(1 Publication)
Species: Hu
Applications: BA

Publications for IFN-alpha H2/IFNA14 Lysate (NBP2-08142) (0)

There are no publications for IFN-alpha H2/IFNA14 Lysate (NBP2-08142).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for IFN-alpha H2/IFNA14 Lysate (NBP2-08142) (0)

There are no reviews for IFN-alpha H2/IFNA14 Lysate (NBP2-08142). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for IFN-alpha H2/IFNA14 Lysate (NBP2-08142) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional IFN-alpha H2/IFNA14 Products

Research Areas for IFN-alpha H2/IFNA14 Lysate (NBP2-08142)

Find related products by research area.

Blogs on IFN-alpha H2/IFNA14

There are no specific blogs for IFN-alpha H2/IFNA14, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our IFN-alpha H2/IFNA14 Overexpression Lysate and receive a gift card or discount.


Bioinformatics

Gene Symbol IFNA14