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ID4 Antibody (3F11)

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Western Blot: ID4 Antibody (3F11) [H00003400-M13] - Analysis of ID4 expression in transfected 293T cell line by ID4 monoclonal antibody (M13), clone 3F11. Lane 1: ID4 transfected lysate (Predicted MW: 16.6 KDa). Lane 2: Non-transfected lysate.
Western Blot: ID4 Antibody (3F11) [H00003400-M13] - Analysis of ID4 expression in transfected 293T cell line by ID4 monoclonal antibody (M13), clone 3F11. Lane 1: ID4 transfected lysate (Predicted MW: 16.6 KDa). Lane 2: ...read more
Sandwich ELISA: ID4 Antibody (3F11) [H00003400-M13] - Detection limit for recombinant GST tagged ID4 is 1 ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clone
3F11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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ID4 Antibody (3F11) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
ID4 (NP_001537.1, 1 a.a. ~ 161 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MKAVSPVRPSGRKAPSGCGGGELALRCLAEHGHSLGGSAAAAAAAAAARCKAAEAAADEPALCLQCDMNDCYSRLRRLVPTIPPNKKVSKVEILQHVIDYILDLQLALETHPALLRQPPPPAPPHHPAGTCPAAPPRTPLTALNTDPAGAVNKQGDSILCR
Specificity
This product is specific for Human ID4 monoclonal antibody (M13), clone 3F11 [Gene ID: 3400].
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
ID4
Purity
IgG purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Sandwich ELISA
  • Western Blot

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for ID4 Antibody (3F11)

  • bHLHb27BHLHB27
  • Class B basic helix-loop-helix protein 27
  • DNA-binding protein inhibitor ID-4
  • IDB4
  • Inhibitor of DNA binding 4
  • inhibitor of DNA binding 4, dominant negative helix-loop-helix protein

Background

Transcription factors containing a basic helix-loop-helix (bHLH) motif regulate expression of tissue-specific genes ina number of mammalian and insect systems. DNA-binding activity of the bHLH proteins is dependent on formation of homo-and/or heterodimers. Dominant-negative HLH proteins encoded by Id-related genes, such as ID4, also contain theHLH-dimerization domain but lack the DNA-binding basic domain. Consequently, Id proteins inhibit binding to DNA andtranscriptional transactivation by heterodimerization with bHLH proteins (Pagliuca et al., 1995 (PubMed7665172)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ID4 Antibody (3F11)
H00003400-M13
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Bioinformatics

Gene Symbol ID4
Uniprot