![Western Blot: ID3 Antibody (2B11) [NB500-112] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Id3 antibody [2B11] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ID3-Antibody-2B11-Western-Blot-NB500-112-img0003.jpg "Western Blot: ID3 Antibody (2B11) [NB500-112] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Id3 antibody [2B11] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IP |
| Clone | 2B11 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | The full-length (amino acids 1-119) mouse ID3 gene expressed in E. coli. |
| Localization | Nuclear |
| Specificity | NB500-112 reacts with human and mouse Id3. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | ID3 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Use in FLOW reported in scientific literature (PMID: 21081927). |
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| Theoretical MW | 14 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: ID3 Antibody (2B11) [NB500-112] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Id3 protein stained by Id3 antibody [2B11] diluted at 1:100. Blue: Hoechst 33342 staining.Scale bar = 10 um.](http://images.novusbio.com/fullsize/ID3-Antibody-2B11-Immunocytochemistry-Immunofluorescence-NB500-112-img0001.jpg "Immunocytochemistry/Immunofluorescence: ID3 Antibody (2B11) [NB500-112] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Id3 protein stained by Id3 antibody [2B11] diluted at 1:100. Blue: Hoechst 33342 staining.Scale bar = 10 um.")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")