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IBRDC2 Antibody (OTI9H10) [Janelia Fluor® 549]

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
OTI9H10
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 549

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IBRDC2 Antibody (OTI9H10) [Janelia Fluor® 549] Summary

Immunogen
Recombinant protein fragment corresponding to amino acids 1-256 of human IBRDC2 (NP_877434) produced in E. coli.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
RNF144B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for IBRDC2 Antibody (OTI9H10) [Janelia Fluor® 549]

  • bA528A10.3
  • E3 ubiquitin-protein ligase RNF144B
  • EC 6.3.2
  • EC 6.3.2.-
  • IBR domain containing 2
  • IBR domain-containing protein 2
  • IBRDC2
  • KIAA0161
  • p53-inducible RING finger protein
  • p53RFP
  • PIR2
  • ring finger protein 144BMGC71786

Background

E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6in the form of a thioester and then directly transfers the ubiquitin to targeted substrates such as LCMT2, therebypromoting their degradation. Induces apoptosis via a TP53/p53-dependent but caspase-independent mechanism

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IBRDC2 Antibody (NBP2-72470JF549) (0)

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Secondary Antibodies

 

Isotype Controls

Additional IBRDC2 Products

Blogs on IBRDC2.

The Latest Research on IBR-type E3 Ubiquitin Ligases
E3 ubiquitin ligases are standards in most antibody catalogs. These proteins are essential to the process of ubiquitination, which is expressed in protein pathways throughout the body and is often linked to disease states. It is widely used as a bioma...  Read full blog post.

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Bioinformatics

Gene Symbol RNF144B