Human Thrombopoietin Quantikine ELISA Kit

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Thrombopoietin (Tpo), is a key regulator of megakaryocytopoiesis and thrombopoiesis. It is principally produced in the liver and is bound and internalized by the receptor Tpo R/c-mpl. Defects in the Tpo-Tpo R signaling pathway are associated with a variety of platelet disorders. The 353 amino acid (aa) human Tpo precursor is cleaved to yield the 332 aa mature protein. Mature human Tpo shares approximately 70% aa sequence homology with mouse and rat Tpo. It is an 80 - 85 kDa protein that consists of an N-terminal domain with homology to Erythropoietin (Epo) and a C-terminal domain that contains multiple N-linked and O-linked glycosylation sites. Tissue specific alternate splicing of human Tpo generates multiple isoforms with internal deletions, insertions, and/or C-terminal substitutions. Tpo promotes the differentiation, proliferation, and maturation of MK and their progenitors. Several other cytokines can promote these functions as well but only in cooperation with Tpo. Notably, IL-3 independently induces MK development, although its effects are restricted to early in the MK lineage. Tpo additionally promotes platelet production, aggregation, ECM adhesion, and activation. It is cleaved by platelet-derived thrombin following Arg191 within the C-terminal domain and subsequently at other sites upon extended digestion. Full length Tpo and shorter forms circulate in the plasma. The C-terminal domain is not required for binding to Tpo R or inducing MK growth and differentiation. Aside from its hematopoietic effects, Tpo is expressed in the brain where it promotes the apoptosis of hypoxia-sensitized neurons and inhibits neuronal differentiation by blocking NGF induced signaling.

Thrombopoietin receptor (Tpo R), also known as myeloproliferative leukemia protein (c-Mpl), is a type I transmembrane protein that is a member of the hematopoietin/cytokine receptor superfamily. As a consequence of alternative splicing, there are four identified mRNA variants. The functional receptor is encoded by the P isoform of mRNA. The human and mouse receptors share approximately 81% amino acid sequence identity. Tpo R is expressed at low levels in various cell types, including hematopoietic progenitor cells, megakaryocytes and platelets.

We have publications tested in 2 confirmed species: Human, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque).

Showing Publications 1 - 10 of 32. Show All 32 Publications. Collapse Publications.

Publications using DTP00B	Applications	Species
SE Samaraweer, T Geukens, DA Casolari, T Nguyen, C Sun, S Bailey, S Moore, J Feng, AW Schreiber, WT Parker, AL Brown, C Butcher, PG Bardy, M Osborn, HS Scott, D Talaulikar, CS Grove, CN Hahn, RJ D'Andrea, DM Ross Novel modes of MPL activation in triple-negative myeloproliferative neoplasms Pathology, 2022-08-05;0(0):. 2022-08-05 [PMID: 36031433] (Human)		Human
T Inano, M Araki, S Morishita, M Imai, Y Kihara, M Okuda, Y Yang, M Ito, S Osaga, H Mano, Y Edahiro, T Ochiai, K Misawa, Y Fukuda, J Ando, N Komatsu Cell-autonomous megakaryopoiesis associated with polyclonal hematopoiesis in triple-negative essential thrombocythemia Scientific Reports, 2021-09-06;11(1):17702. 2021-09-06 [PMID: 34489506] (Human)		Human
GA Lavranou, S Mentzelopo, P Katsaounou, I Siempos, I Kalomenidi, A Geranaki, C Routsi, S Zakynthino Can Coagulation System Disorders and Cytokine and Inflammatory Marker Levels Predict the Temporary Clinical Deterioration or Improvement of Septic Patients on ICU Admission? Journal of Clinical Medicine, 2021-04-07;10(8):. 2021-04-07 [PMID: 33917002] (Human)		Human
Z Herold, M Dank, M Herold, P Nagy, K Rosta, A Somogyi Histopathological Chromogranin A-Positivity Is Associated with Right-Sided Colorectal Cancers and Worse Prognosis Cancers, 2020-12-29;13(1):. 2020-12-29 [PMID: 33383764] (Human)		Human
T Fukuda, M Hamaguchi, T Osaka, Y Hashimoto, E Ushigome, M Asano, M Yamazaki, E Fukuda, K Yamaguchi, K Ogawa, N Goshima, M Fukui A Pilot Study on the Effect of Anti-Thrombopoietin Antibody on Platelet Count in Patients with Type 2 Diabetes Molecules, 2020-04-04;25(7):. 2020-04-04 [PMID: 32260359] (Human)		Human
T Sun, M Ju, X Dai, H Dong, W Gu, Y Gao, R Fu, X Liu, Y Huang, W Liu, Y Chi, W Wang, H Li, Y Zhou, L Shi, R Yang, L Zhang Multilevel defects in the hematopoietic niche in essential thrombocythemia Haematologica, 2019-07-09;0(0):. 2019-07-09 [PMID: 31289202] (Human)		Human
C Zaninetti, P Gresele, A Bertomoro, C Klersy, E De Candia, D Veneri, S Barozzi, T Fierro, MA Alberelli, V Musella, P Noris, F Fabris, CL Balduini, A Pecci Eltrombopag for the treatment of inherited thrombocytopenias: a phase 2 clinical trial Haematologica, 2019-07-04;0(0):. 2019-07-04 [PMID: 31273088] (Human)		Human
Y Matsukiyo, H Nagai, T Matsui, Y Igarashi Host Immunological Effects of Partial Splenic Embolization in Patients with Liver Cirrhosis J Immunol Res, 2018-07-15;2018(0):1746391. 2018-07-15 [PMID: 30116748] (Human)		Human
M Germeshaus, P Ancliff, J Estrada, M Metzler, E Ponstingl, H Rütschle, D Schwabe, RH Scott, S Unal, A Wawer, B Zeller, M Ballmaier MECOM-associated syndrome: a heterogeneous inherited bone marrow failure syndrome with amegakaryocytic thrombocytopenia Blood Adv, 2018-03-27;2(6):586-596. 2018-03-27 [PMID: 29540340] (Human)		Human
CPM Hayward, S Tasneem, GE Rivard Thrombopoietin levels in Quebec platelet disorder-Implications for the mechanism of thrombocytopenia Int J Lab Hematol, 2018-02-01;0(0):. 2018-02-01 [PMID: 29388746] (Human)		Human
S Li, J Shao, M Xia, N Zhang, J Yang, H Li, H Jiang Thrombopoietin and its receptor expression in pediatric patients with chronic immune thrombocytopenia Hematology, 2018-01-09;0(0):1-6. 2018-01-09 [PMID: 29313460] (Human)		Human
S Ichimata, M Kobayashi, K Honda, S Shibata, A Matsumoto, H Kanno Acquired amegakaryocytic thrombocytopenia previously diagnosed as idiopathic thrombocytopenic purpura in a patient with hepatitis C virus infection World J. Gastroenterol., 2017-09-21;23(35):6540-6545. 2017-09-21 [PMID: 29085203] (Human)		Human
A Seo, M Ben-Harosh, M Sirin, J Stein, O Dgany, J Kaplelushn, M Hoenig, U Pannicke, M Lorenz, K Schwarz, C Stockklaus, T Walsh, S Gulsuner, MK Lee, A Sendamarai, M Sanchez-Bo, MC King, H Cario, AE Kulozik, KM Debatin, A Schulz, H Tamary, A Shimamura Bone marrow failure unresponsive to bone marrow transplant is caused by mutations in THPO Blood, 2017-05-30;0(0):. 2017-05-30 [PMID: 28559357] (Human)		Human
P Noris, C Marconi, D De Rocco, F Melazzini, T Pippucci, G Loffredo, T Giangregor, A Pecci, M Seri, A Savoia A new form of inherited thrombocytopenia due to monoallelic loss of function mutation in the thrombopoietin gene Br. J. Haematol., 2017-05-03;0(0):. 2017-05-03 [PMID: 28466964] (Human)		Human
S Besbes, S Shah, I Al-Dybiat, S Mirshahi, H Helfer, H Najah, C Fourgeaud, M Pocard, I Ghedira, J Soria, M Mirshahi Thrombopoietin Secretion by Human Ovarian Cancer Cells Int J Cell Biol, 2017-03-30;2017(0):1873834. 2017-03-30 [PMID: 28465688] (Human)		Human
L Desbourdes, J Javary, T Charbonnie, N Ishac, J Bourgeais, A Iltis, JC Chomel, A Turhan, F Guilloton, K Tarte, MV Demattei, E Ducrocq, F Rouleux-Bo, E Gyan, O Hérault, J Domenech Alteration Analysis of Bone Marrow Mesenchymal Stromal Cells from De Novo Acute Myeloid Leukemia Patients at Diagnosis Stem Cells Dev., 2017-04-10;0(0):. 2017-04-10 [PMID: 28394200] (Human)		Human
Outi Laine Elevated thrombopoietin and platelet indices confirm active thrombopoiesis but fail to predict clinical severity of puumala hantavirus infection Medicine (Baltimore), 2016-12-01;95(52):e5689. 2016-12-01 [PMID: 28033261] (Human)		Human
Jizhou Zhang Persistent elevated bone marrow plasma levels of thrombopoietin in patients with aplastic anemia Cytokine, 2016-06-03;85(0):11-13. 2016-06-03 [PMID: 27269179] (Human)		Human
Haegele , Stefanie, Offensperger , Florian, Pereyra , David, Lahner , Elisabet, Assinger , Alice, Fleischmann , Edith, Gruenberger , Birgit, Gruenberger , Thomas, Brostjan , Christin, Starlinger , Patrick Deficiency in thrombopoietin induction after liver surgery is associated with postoperative liver dysfunction. PLoS ONE, 2015-01-22;10(1):e0116985. 2015-01-22 [PMID: 25611592] (Human)		Human
de Mast Q, de Groot PG, van Heerde WL, Roestenberg M, van Velzen JF, Verbruggen B, Roest M, McCall M, Nieman AE, Westendorp J, Syafruddin D, Fijnheer R, van Dongen-Lases EC, Sauerwein RW, van der Ven AJ Thrombocytopenia in early malaria is associated with GP1b shedding in absence of systemic platelet activation and consumptive coagulopathy. Br. J. Haematol., 2010-10-19;151(5):495-503. 2010-10-19 [PMID: 20955404] (Human)		Human
Pecci A, Gresele P, Klersy C, Savoia A, Noris P, Fierro T, Bozzi V, Mezzasoma AM, Melazzini F, Balduini CL Eltrombopag for the treatment of the inherited thrombocytopenia deriving from MYH9 mutations. Blood, 2010-09-15;116(26):5832-7. 2010-09-15 [PMID: 20844233] (Human)		Human
Yang L, Wang L, Zhao CH, Zhu XJ, Hou Y, Jun P, Hou M Contributions of TRAIL-mediated megakaryocyte apoptosis to impaired megakaryocyte and platelet production in immune thrombocytopenia. Blood, 2010-07-29;116(20):4307-16. 2010-07-29 [PMID: 20671119] (Human)		Human
Glembotsky AC, Korin L, Lev PR, Chazarreta CD, Marta RF, Molinas FC, Heller PG Screening for MPL mutations in essential thrombocythemia and primary myelofibrosis: normal Mpl expression and absence of constitutive STAT3 and STAT5 activation in MPLW515L-positive platelets. Eur. J. Haematol., 2010-01-22;84(5):398-405. 2010-01-22 [PMID: 20113333] (Human)		Human
Lupia E, Bosco O, Goffi A Thrombopoietin contributes to enhanced platelet activation in cigarette smokers. Atherosclerosis, 2009-11-20;210(1):314-9. 2009-11-20 [PMID: 20006331] (Human)		Human
Liem-Moolenaar M, Cerneus D, Molloy C, End D, Brown K, de Kam M, Cohen A, van Hensbergen Y, Burggraaf J Pharmacodynamics and Pharmacokinetics of the Novel Thrombopoietin Mimetic Peptide RWJ-800088 in Humans. 2008-10-01;84(0):481. 2008-10-01 [PMID: 19238653] (Human)		Human
El-Harith el-HA, Roesl C, Ballmaier M, Germeshausen M, Frye-Boukhriss H, von Neuhoff N, Becker C, Nurnberg G, Nurnberg P, Ahmed MA, Hubener J, Schmidtke J, Welte K, Stuhrmann M Familial thrombocytosis caused by the novel germ-line mutation p.Pro106Leu in the MPL gene. Br. J. Haematol., 2008-11-19;144(2):185-94. 2008-11-19 [PMID: 19036112] (Human)		Human
Asahi A, Nishimoto T, Okazaki Y, Suzuki H, Masaoka T, Kawakami Y, Ikeda Y, Kuwana M Helicobacter pylori eradication shifts monocyte Fcgamma receptor balance toward inhibitory FcgammaRIIB in immune thrombocytopenic purpura patients. J. Clin. Invest., 2008-08-01;118(8):2939-49. 2008-08-01 [PMID: 18654664] (Human)		Human
Molica S, Vitelli G, Cutrona G, Todoerti K, Mirabelli R, Digiesi G, Morabito F, Neri A, Ferrarini M Serum thrombopoietin compared with ZAP-70 and immunoglobulin heavy-chain gene mutation status as a predictor of time to first treatment in early chronic lymphocytic leukemia. Leuk. Lymphoma, 2008-01-01;49(1):62-7. 2008-01-01 [PMID: 18203013] (Human)		Human
Reinhold A, Zhang J, Gessner R, Felderhoff-Mueser U, Obladen M, Dame C High thrombopoietin concentrations in the cerebrospinal fluid of neonates with sepsis and intraventricular hemorrhage may contribute to brain damage. J. Interferon Cytokine Res., 2007-02-01;27(2):137-45. 2007-02-01 [PMID: 17316141] (Human)		Human
D'Hondt S Circulating thrombopoietin as an in vivo growth factor for blast cells in acute myeloid leukemia. Blood, 2005-11-29;107(6):2525-30. 2005-11-29 [PMID: 16317100] (Human)		Human
Drouet M, Mourcin F, Grenier N, Leroux V, Denis J, Mayol JF, Thullier P, Lataillade JJ, Herodin F Single administration of stem cell factor, FLT-3 ligand, megakaryocyte growth and development factor, and interleukin-3 in combination soon after irradiation prevents nonhuman primates from myelosuppression: long-term follow-up of hematopoiesis. Blood, 2003-10-02;103(3):878-85. 2003-10-02 [PMID: 14525791] (Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque))		Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
Solanilla A, Dechanet J, El Andaloussi A, Dupouy M, Godard F, Chabrol J, Charbord P, Reiffers J, Nurden AT, Weksler B, Moreau JF, Ripoche J CD40-ligand stimulates myelopoiesis by regulating flt3-ligand and thrombopoietin production in bone marrow stromal cells. Blood, 2000-06-15;95(12):3758-64. 2000-06-15 [PMID: 10845907] (Human)		Human
Show All 32 Publications. Collapse Publications.

Array DTP00B

• Thrombopoietin/THPO Antibodies
• Thrombopoietin/THPO ELISA Kit
• Thrombopoietin/THPO Lysate
• Thrombopoietin/THPO Protein
• Thrombopoietin/THPO Proteins and Enzymes
• Thrombopoietin/THPO ELISAs