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HNF-3 alpha/FoxA1 Antibody (654126) [Unconjugated]

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Western blot shows lysates of LNCaP human prostate cancer cell line. Gels were loaded with 30 µg of cytoplasmic (Cyto) and 30 µg of nuclear extracts (Nuc). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human HNF-3a/FoxA1 Monoclonal Antibody (Catalog # MAB6778) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for HNF-3a/FoxA1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of LNCaP human prostate cancer cell line. Gels were loaded with 30 µg of cytoplasmic (Cyto) and 30 µg of nuclear extracts (Nuc). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human ...read more
HNF-3a/FoxA1 was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human HNF-3a/FoxA1 Monoclonal Antibody (Catalog # MAB6778) at 10 µg/mL for 3 hours at room temperature. Cells were ...read more
HNF‑3 alpha /FoxA1 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human HNF‑3 alpha /FoxA1 Monoclonal Antibody (Catalog # MAB6778) at 1.7 µg/mL ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, ICC/IF
Clone
654126
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB6778-SP
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HNF-3 alpha/FoxA1 Antibody (654126) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human HNF‑3 alpha /FoxA1
Met253-Ser472
Accession # P55317
Specificity
Detects human HNF‑3 alpha /FoxA1 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
FOXA1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 1-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HNF-3 alpha/FoxA1 Antibody (654126) [Unconjugated]

  • forkhead box A1
  • Forkhead box protein A1
  • FoxA1
  • hepatocyte nuclear factor 3, alpha
  • hepatocyte nuclear factor 3-alpha
  • HNF3 alpha
  • HNF-3 alpha
  • HNF-3A
  • HNF-3-alpha
  • HNF3ATCF-3A
  • MGC33105
  • TCF3A
  • Transcription factor 3A

Background

FoxA1 (forkhead box A1), also called HNF-3 alpha (hepatocyte nuclear factor 3 alpha) or TCF3A (transcription factor 3A) is a 473 amino acid (aa), 49 kDa (predicted) protein of the forkhead nuclear transcription factor family. It regulates transcription downstream of the estrogen receptor and other steroid hormone receptors. FoxA1 is expressed in breast luminal cells and correlates with proliferation in breast cancer cell lines. Within the sequence used as an immunogen, human FoxA1 shares 93% and 86% aa identity with mouse and rat FoxA1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol FOXA1
Uniprot