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Histone H3.1 [Monomethyl Arg2] Antibody (4G5)

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Western Blot: Histone H3.1 [Monomethyl Arg2] Antibody (4G5) [NBP3-26130] - Positive Western Blot detected in Hela whole cell lysate. HepG2 whole cell lysate. SH-SY5Y whole cell lysate. All lanes: Histone H3.1 ...read more
Immunocytochemistry/ Immunofluorescence: Histone H3.1 [Monomethyl Arg2] Antibody (4G5) [NBP3-26130] - Staining of Hela cells with Histone H3.1 [Monomethyl Arg2] Antibody (4G5) at 1:60, counter-stained with DAPI. The ...read more
Immunocytochemistry/ Immunofluorescence: Histone H3.1 [Monomethyl Arg2] Antibody (4G5) [NBP3-26130] - Analysis of Histone H3.1 [Monomethyl Arg2] Antibody (4G5) diluted at 1:100 and staining in Hela cells. After dewaxing ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clone
4G5
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Histone H3.1 [Monomethyl Arg2] Antibody (4G5) Summary

Additional Information
Recombinant monoclonal antibody expressed in HEK293F cells
Immunogen
Synthesized peptide [UniProt P68431]
Modification
Monomethyl Arg2
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
HIST1H3E
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:50-1:500
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for Histone H3.1 [Monomethyl Arg2] Antibody (4G5)

  • H3 histone family, member D
  • H3.1HIST1H3D
  • H3/d
  • H3FA
  • H3FB
  • H3FC
  • H3FDHIST1H3F
  • H3FF
  • H3FH
  • H3FI
  • H3FJ
  • H3FK
  • H3FL
  • HIST1H3A
  • HIST1H3B
  • HIST1H3C
  • HIST1H3G
  • HIST1H3H
  • HIST1H3I
  • HIST1H3J
  • histone 1, H3e
  • histone cluster 1, H3e
  • Histone H3.1
  • Histone H3/a
  • Histone H3/b
  • Histone H3/c
  • Histone H3/d
  • Histone H3/f
  • Histone H3/h
  • Histone H3/i
  • Histone H3/j
  • Histone H3/k
  • Histone H3/l

Background

In eukaryotes, DNA is wrapped around histone octamers to form the basic unit of chromatin structure. The octamer is composed of Histones H2A, H2B, H3 and H4, and it associates with approximately 200 base pairs of DNA to form the nucleosome. The association of DNA with histones results in dense packing of chromatin, which restricts proteins involved in gene transcription from binding to DNA. Histone H3, the core protein of the nucleosome, becomes phosphorylated at the end of prophase. The two major sites of phosphorylation are the mitosis-specific site Ser 10 and Ser 28, both of which are extensively phosphorylated in DNA-bound forms of Histone H3 and in nucleosomal Histone H3. The nucleosome structure of Histone H3 promotes N-terminal phosphorylation in vitro. Phosphorylation of Histone H3 at Ser 10 is an essenti

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Histone H3.1 Antibody (NBP3-26130) (0)

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Secondary Antibodies

 

Isotype Controls

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Research Areas for Histone H3.1 Antibody (NBP3-26130)

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Blogs on Histone H3.1.

H3.1 - A core histone essential for genome storage and organization
Histones are the main protein component of chromatin and are essential for the storage and compaction of the genome. DNA wraps around histone oligomers to make up nucleosomes, the individual subunits of chromatin. By altering the accessibility of t...  Read full blog post.

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Bioinformatics

Gene Symbol HIST1H3E