![Western Blot: Histone H3.1 [Monomethyl Lys18] Antibody (2B5) [NBP3-26134] - Positive Western Blot detected in MCF-7 whole cell lysate. Hela whole cell lysate. HepG2 whole cell lysate. SH-SY5Y whole cell lysate, Rat brain tissue. All lanes: Histone H3.1 [Monomethyl Lys18] Antibody at 0.7ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 15 KDa Observed band size: 15 KDa](http://images.novusbio.com/fullsize/nbp3-26134_rabbit-histone-h3-1-monomethyl-lys-18-mab-2b5-28220241140934.jpg "Western Blot: Histone H3.1 [Monomethyl Lys18] Antibody (2B5) [NBP3-26134] - Positive Western Blot detected in MCF-7 whole cell lysate. Hela whole cell lysate. HepG2 whole cell lysate. SH-SY5Y whole cell lysate, Rat brain tissue. All lanes: Histone H3.1 [Monomethyl Lys18] Antibody at 0.7ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 15 KDa Observed band size: 15 KDa")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF |
| Clone | 2B5 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Additional Information | Recombinant monoclonal antibody expressed in HEK293F cells |
| Immunogen | Synthesized peptide [UniProt P68431] |
| Modification | Monomethyl Lys18 |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | HIST1H3E |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at -20 to -70C. Avoid freeze-thaw cycles. |
| Buffer | PBS, pH 7.4, 150mM NaCl and 50% glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Affinity purified |
![Immunohistochemistry Histone H2AE [Hydroxy Tyr39] Antibody (2J2Q7)](https://images.novusbio.com/images/nbp3-33534_rabbit-histone-h2ae-hydroxy-tyr39-mab-2j2q7-1012202411334085.jpg)
![Western Blot Histone H2AE [Hydroxy Tyr39] Antibody (2J2Q7)](https://images.novusbio.com/images/nbp3-33534_rabbit-histone-h2ae-hydroxy-tyr39-mab-2j2q7-1012202411343397.jpg)
![Western Blot Histone H2AE [Hydroxy Tyr39] Antibody (2J2Q7)](https://images.novusbio.com/images/nbp3-33534_rabbit-histone-h2ae-hydroxy-tyr39-mab-2j2q7-1012202411404011.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for Histone H3.1 Antibody (NBP3-26134)Find related products by research area.
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H3.1 - A core histone essential for genome storage and organization Histones are the main protein component of chromatin and are essential for the storage and compaction of the genome. DNA wraps around histone oligomers to make up nucleosomes, the individual subunits of chromatin. By altering the accessibility of t... Read full blog post. |
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| Gene Symbol | HIST1H3E |
![Immunocytochemistry/Immunofluorescence: Histone H3.1 [Monomethyl Lys18] Antibody (2B5) [NBP3-26134] - Analysis of Histone H3.1 [Monomethyl Lys18] Antibody (2B5) diluted at 1:100 and staining in Hela cells. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26134_rabbit-histone-h3-1-monomethyl-lys-18-mab-2b5-2822024132360.jpg "Immunocytochemistry/Immunofluorescence: Histone H3.1 [Monomethyl Lys18] Antibody (2B5) [NBP3-26134] - Analysis of Histone H3.1 [Monomethyl Lys18] Antibody (2B5) diluted at 1:100 and staining in Hela cells. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.")
![Immunocytochemistry/Immunofluorescence: Histone H3.1 [Monomethyl Lys18] Antibody (2B5) [NBP3-26134] - Staining of Hela cells with Histone H3.1 [Monomethyl Lys18] Antibody (2B5) at 1:43, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26134_rabbit-histone-h3-1-monomethyl-lys-18-mab-2b5-28220241312240.jpg "Immunocytochemistry/Immunofluorescence: Histone H3.1 [Monomethyl Lys18] Antibody (2B5) [NBP3-26134] - Staining of Hela cells with Histone H3.1 [Monomethyl Lys18] Antibody (2B5) at 1:43, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")