![Western Blot: Histone H1 Antibody (HH1/957) - Azide and BSA Free [NBP2-47754] - Western Blot Analysis of human heart tissue lysate using Histone H1 Antibody (HH1/957).](http://images.novusbio.com/fullsize/Histone-H1-Antibody-HH1-957-Azide-and-BSA-Free-Western-Blot-NBP2-47754-img0010.jpg "Western Blot: Histone H1 Antibody (HH1/957) - Azide and BSA Free [NBP2-47754] - Western Blot Analysis of human heart tissue lysate using Histone H1 Antibody (HH1/957).")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, CyTOF-ready, ICC/IF |
| Clone | HH1/957 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1.0 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 1mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-45184). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. |
| Immunogen | Recombinant full-length human H1F0 Histone H1.0 protein |
| Localization | Nuclear |
| Marker | Pan Nuclear Marker |
| Isotype | IgG2a Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | H1-1 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Theoretical MW | 30 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 1mM PBS |
| Preservative | No Preservative |
| Concentration | 1.0 mg/ml |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Histone H1 Antibody (NBP2-47754)Find related products by research area.
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Histone H3 Eukaryotic chromosomes are formed through the highly organized and structural wrapping of DNA genetic material around histone proteins into the classic "bead on a string" globular structure of nucleosomes. The histone family consists of five family me... Read full blog post. |
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| Gene Symbol | H1-1 |
![Immunocytochemistry/Immunofluorescence: Histone H1 Antibody (HH1/957) - Azide and BSA Free [NBP2-47754] - Human cell culture cells were fixed with paraformaldehyde, permeabilized and incubated 1 hour at RT with primary antibody. This image was submitted via customer review.](http://images.novusbio.com/fullsize/Histone-H1-Antibody-HH1-957-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-47754-img0002.jpg "Immunocytochemistry/Immunofluorescence: Histone H1 Antibody (HH1/957) - Azide and BSA Free [NBP2-47754] - Human cell culture cells were fixed with paraformaldehyde, permeabilized and incubated 1 hour at RT with primary antibody. This image was submitted via customer review.")
![Immunohistochemistry-Paraffin: Histone H1 Antibody (HH1/957) - Azide and BSA Free [NBP2-47754] - Formalin-fixed, paraffin-embedded human Pancreas stained with Histone H1 Antibody (HH1/957).](http://images.novusbio.com/fullsize/Histone-H1-Antibody-HH1-957-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47754-img0011.jpg "Immunohistochemistry-Paraffin: Histone H1 Antibody (HH1/957) - Azide and BSA Free [NBP2-47754] - Formalin-fixed, paraffin-embedded human Pancreas stained with Histone H1 Antibody (HH1/957).")
![Flow Cytometry: Histone H1 Antibody (HH1/957) - Azide and BSA Free [NBP2-47754] - Flow Cytometric Analysis of HeLa cells. Histone H1 Mouse Monoclonal Antibody (HH1/957) followed by goat anti-mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/Histone-H1-Antibody-HH1-957-Azide-and-BSA-Free-Flow-Cytometry-NBP2-47754-img0009.jpg "Flow Cytometry: Histone H1 Antibody (HH1/957) - Azide and BSA Free [NBP2-47754] - Flow Cytometric Analysis of HeLa cells. Histone H1 Mouse Monoclonal Antibody (HH1/957) followed by goat anti-mouse IgG-CF488 (Blue); Isotype Control (Red).")
![Immunocytochemistry/Immunofluorescence: Histone H1 Antibody (HH1/957) - Azide and BSA Free [NBP2-47754] - Confocal Immunofluorescence image of HeLa cells using Green (CF488) and Phalloidin is used to label the membranes.](http://images.novusbio.com/fullsize/Histone-H1-Antibody-HH1-957-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-47754-img0001.jpg "Immunocytochemistry/Immunofluorescence: Histone H1 Antibody (HH1/957) - Azide and BSA Free [NBP2-47754] - Confocal Immunofluorescence image of HeLa cells using Green (CF488) and Phalloidin is used to label the membranes.")
![Immunohistochemistry-Paraffin: Histone H1 Antibody (HH1/957) - Azide and BSA Free [NBP2-47754] - Human Tonsil stained with Histone H1 Monoclonal Antibody (HH1/957)](http://images.novusbio.com/fullsize/Histone-H1-Antibody-HH1-957-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47754-img0003.jpg "Immunohistochemistry-Paraffin: Histone H1 Antibody (HH1/957) - Azide and BSA Free [NBP2-47754] - Human Tonsil stained with Histone H1 Monoclonal Antibody (HH1/957)")
![Immunohistochemistry-Paraffin: Histone H1 Antibody (HH1/957) - Azide and BSA Free [NBP2-47754] - Formalin-fixed, paraffin-embedded human Angiosarcoma stained with Histone H1 Antibody (HH1/957).](http://images.novusbio.com/fullsize/Histone-H1-Antibody-HH1-957-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47754-img0004.jpg "Immunohistochemistry-Paraffin: Histone H1 Antibody (HH1/957) - Azide and BSA Free [NBP2-47754] - Formalin-fixed, paraffin-embedded human Angiosarcoma stained with Histone H1 Antibody (HH1/957).")
![Immunohistochemistry-Paraffin: Histone H1 Antibody (HH1/957) - Azide and BSA Free [NBP2-47754] - Human Ovarian Carcinoma stained with Histone H1 Monoclonal Antibody (HH1/957)](http://images.novusbio.com/fullsize/Histone-H1-Antibody-HH1-957-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47754-img0005.jpg "Immunohistochemistry-Paraffin: Histone H1 Antibody (HH1/957) - Azide and BSA Free [NBP2-47754] - Human Ovarian Carcinoma stained with Histone H1 Monoclonal Antibody (HH1/957)")
![Immunohistochemistry-Paraffin: Histone H1 Antibody (HH1/957) - Azide and BSA Free [NBP2-47754] - Human Tonsil stained with Histone H1 Monoclonal Antibody (HH1/957)](http://images.novusbio.com/fullsize/Histone-H1-Antibody-HH1-957-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47754-img0006.jpg "Immunohistochemistry-Paraffin: Histone H1 Antibody (HH1/957) - Azide and BSA Free [NBP2-47754] - Human Tonsil stained with Histone H1 Monoclonal Antibody (HH1/957)")
![Immunohistochemistry-Paraffin: Histone H1 Antibody (HH1/957) - Azide and BSA Free [NBP2-47754] - Rat Pancreas stained with Histone H1 Monoclonal Antibody (HH1/957)](http://images.novusbio.com/fullsize/Histone-H1-Antibody-HH1-957-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47754-img0007.jpg "Immunohistochemistry-Paraffin: Histone H1 Antibody (HH1/957) - Azide and BSA Free [NBP2-47754] - Rat Pancreas stained with Histone H1 Monoclonal Antibody (HH1/957)")
![Immunohistochemistry-Paraffin: Histone H1 Antibody (HH1/957) - Azide and BSA Free [NBP2-47754] - Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Histone H1 Antibody (HH1/957).](http://images.novusbio.com/fullsize/Histone-H1-Antibody-HH1-957-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47754-img0008.jpg "Immunohistochemistry-Paraffin: Histone H1 Antibody (HH1/957) - Azide and BSA Free [NBP2-47754] - Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Histone H1 Antibody (HH1/957).")
