Recombinant Human GSTM2 His Protein Summary
Description |
A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-218 of Human GSTM2 Source: E.coli Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MPMTLGYWNI RGLAHSIRLL LEYTDSSYEE KKYTMGDAPD YDRSQWLNEK FKLGLDFPNL PYLIDGTHKI TQSNAILRYI ARKHNLCGES EKEQIREDIL ENQFMDSRMQ LAKLCYDPDF EKLKPEYLQA LPEMLKLYSQ FLGKQPWFLG DKITFVDFIA YDVLERNQVF EPSCLDAFPN LKDFISRFEG LEKISAYMKS SRFLPRPVFT KMAVWGNK |
Details of Functionality |
Specific activity is > 250 units/mg, and is defined as the amount of enzyme that conjugate 1.0 umole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C. |
Source |
E. coli |
Protein/Peptide Type |
Recombinant Protein |
Gene |
GSTM2 |
Purity |
>95%, by SDS-PAGE |
Applications/Dilutions
Dilutions |
|
Theoretical MW |
27.9 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Buffer |
20 mM Tris-HCl buffer (pH8.0), 10% glycerol, 0.1 M NaCl, 1 mM DTT |
Preservative |
No Preservative |
Concentration |
0.5 mg/ml |
Purity |
>95%, by SDS-PAGE |
Alternate Names for Recombinant Human GSTM2 His Protein
Background
GSTM2, also known as Glutathione S-transferase Mu 2, is member of the glutathione s-transferase (GST) family of proteins. There are eight families of GST proteins, namely alpha, kappa, mu, omega, pi, sigma, theta and zeta, each of which is composed of proteins that have a variety of functions throughout the cell. The mu class of enzymes functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. Recombinant human GSTM2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are
guaranteed for 3 months from date of receipt.
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