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GST Epitope Tag Antibody (3G10)

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Western Blot: GST Epitope Tag Antibody (3G10) [NB600-446] - A. 2 ug His-recombinant protein B. 2 ug GST-recombinant protein C. 1 ug GST-recombinant protein D. 0.5 ug GST-recombinant protein 10 % SDS-PAGE GST tag ...read more
Western Blot: GST Epitope Tag Antibody (3G10) [NB600-446] - A. Non-induced cell lysate from E. coli expressing GST-tagged proteinsB. IPTG-induced cell lysate from E. coli expressing GST-tagged proteins10 % SDS-PAGE GST ...read more

Product Details

Summary
Reactivity ETSpecies Glossary
Applications WB, IP
Clone
3G10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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GST Epitope Tag Antibody (3G10) Summary

Immunogen
GST protein of the parasitic helminth Schistosoma japonicum expressed in E. coli
Specificity
Recognizes Glutathione S-Transferase (GST), a 26-kDa protein encoded by the parasitic helminth Schistosoma japonicum.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
GSTM1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 0.1-2 ug/ml
Publications
Read Publications using NB600-446.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Purity
Protein G purified

Alternate Names for GST Epitope Tag Antibody (3G10)

  • EC 2.5.1.18
  • glutathione S-alkyltransferase
  • glutathione S-aralkyltransferase
  • glutathione S-aryltransferase
  • glutathione S-transferase M1
  • glutathione S-transferase mu 1
  • GST class-mu 1
  • GST HB subunit 4
  • GST1
  • GSTM1-1
  • GSTM1a-1a
  • GSTM1b-1b
  • GTH4
  • GTM1
  • HB subunit 4
  • H-B
  • MGC26563
  • MU
  • MU-1
  • S-(hydroxyalkyl)glutathione lyase

Background

Glutathione S transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunological, and structural properties, the soluble human GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. The amino acid sequence GST is highly conserved in most organisms, including mammals.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GST Epitope Tag Antibody (NB600-446)(9)

We have publications tested in 2 applications: IP, WB.


Filter By Application
IP
(2)
WB
(4)
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Filter By Species
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Showing Publications 1 - 9 of 9.
Publications using NB600-446 Applications Species
Liu Y, McDonald N, Naegele S et al. The F-BAR Domain of Rga7 Relies on a Cooperative Mechanism of Membrane Binding with a Partner Protein during Fission Yeast Cytokinesis Cell Reports 2019-03-01 [PMID: 30840879] (WB) WB
Miller KE, Lo WC, Lee ME et al. Fine-tuning the orientation of the polarity axis by Rga1, a Cdc42 GTPase activating protein. Mol. Biol. Cell. 2017-10-26 [PMID: 29074565] (WB) WB
Di XJ, Wang YJ, Han DY et al. Grp94 Delivers gamma-aminobutyric Acid Type A (GABAA) Receptors to Hrd1-Mediatd Endoplasmic Reticulum-Associated Degradation. J. Biol. Chem. 2016-03-04 [PMID: 26945068]
Wang N, Wang M, Zhu Yh et al. The Rho-GEF Gef3 interacts with the septin complex and activates the GTPase Rho4 during fission yeast cytokinesis. Mol. Biol. Cell. 2014-11-19 [PMID: 25411334]
Zhu YH, Ye Y, Wu Z et al. Cooperation between Rho-GEF Gef2 and its binding partner Nod1 in the regulation of fission yeast cytokinesis Mol Biol Cell 2013-08-23 [PMID: 23966468] (WB) WB
Laporte D, Coffman VC, Lee IJ, Wu JQ. Assembly and architecture of precursor nodes during fission yeast cytokinesis. J Cell Biol 2011-03-21 [PMID: 21422229]
Siepe D, Jentsch S. Prolyl isomerase Pin1 acts as a switch to control the degree of substrate ubiquitylation. Nat Cell Biol 2009-08-01 [PMID: 19597489] (IP) IP
Luque CM, Perez-Ferreiro CM, Perez-Gonzalez A et al. An alternative domain containing a leucine-rich sequence regulates nuclear cytoplasmic localization of protein 4.1R. J Biol Chem 2003-01-01 [PMID: 12427749] (IP) IP
Miller KE Negative Regulation of Polarity Establishment in Saccharomyces cerevisiae Thesis 2019-01-01 (WB) WB

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Product General Protocols

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Video Protocols

WB Video Protocol

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Bioinformatics

Gene Symbol GSTM1
Uniprot