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GREB1 Antibody (CL13863)

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Staining of human small intestine shows no positivity in glandular cells as expected.
Staining of human small intestine shows no positivity in glandular cells as expected.
Staining in U2OS cell line with Anti-GREB1 monoclonal antibody, showing specific staining of nucleoplasm and cytosol in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where ...read more
Staining of human prostate cancer shows strong nuclear positivity in tumor cells.
Staining of human fallopian tube shows weak to moderate cytoplasmic and nuclear positivity in glandular cells.
Staining of human ovarian cancer (sex cord tumor) shows strong nuclear positivity in tumor cells.
Staining of human endometrium shows moderate cytoplasmic positivity in glandular cells.
Staining of human ovarian cancer (granulosa cell tumor) shows moderate nuclear positivity in tumor cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF, IHC
Clone
CL13863
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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GREB1 Antibody (CL13863) Summary

Immunogen
This antibody was designed to target the recombinant protein GREB1, with the precise immunogen sequence being withheld for proprietary reasons.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
GREB1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
Application Notes
For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments, For ICC/IF, we recommend using a combination of PFA and Triton X-100. This will give you the optimal results for your experiments.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.2, 40% glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for GREB1 Antibody (CL13863)

  • gene regulated by estrogen in breast cancer protein
  • growth regulation by estrogen in breast cancer 1
  • KIAA0575Gene regulated in breast cancer 1 protein
  • protein GREB1

Background

GREB1 is an estrogen-responsive gene that is an early response gene in the estrogen receptor-regulated pathway. It is thought to play an important role in hormone-responsive tissues and cancer. Three alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq]. Transcript Variant: This variant (a) encodes the longest isoform (a).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GREB1