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Glucose 6 phosphate isomerase Antibody (CPTC-GPI-1) - Azide and BSA Free

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Western Blot: Glucose 6 phosphate isomerase Antibody (CPTC-GPI-1) - Azide and BSA Free [NBP2-79845] - Western Blot Analysis of T47D, PC3, HePG2 cell lysates using Glucose 6 phosphate isomerase Antibody (CPTC-GPI-1).
Immunocytochemistry/ Immunofluorescence: Glucose 6 phosphate isomerase Antibody (CPTC-GPI-1) - Azide and BSA Free [NBP2-79845] - Immunofluorescence Analysis of human MCF-7 cells labeling GPI with Glucose 6 phosphate ...read more
Immunohistochemistry-Paraffin: Glucose 6 phosphate isomerase Antibody (CPTC-GPI-1) - Azide and BSA Free [NBP2-79845] - Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with Glucose 6 phosphate isomerase ...read more
Protein Array: Glucose 6 phosphate isomerase Antibody (CPTC-GPI-1) - Azide and BSA Free [NBP2-79845] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Glucose 6 phosphate isomerase ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, MA
Clone
CPTC-GPI-1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1 mg/ml

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Glucose 6 phosphate isomerase Antibody (CPTC-GPI-1) - Azide and BSA Free Summary

Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-79711).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Immunogen
Recombinant human full-length Glucose 6 phosphate isomerase protein (Uniprot: P06744)
Localization
Cytoplasm (secreted)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
GPI
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry 1-2 ug/ml
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Protein Array
  • Western Blot 1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
10 mM PBS
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for Glucose 6 phosphate isomerase Antibody (CPTC-GPI-1) - Azide and BSA Free

  • AMFGNPI
  • Autocrine motility factor
  • DKFZp686C13233
  • EC 5.3.1.9
  • glucose phosphate isomerase
  • glucose-6-phosphate isomerase
  • hexose monophosphate isomerase
  • hexosephosphate isomerase
  • neuroleukin
  • NLKSA36
  • oxoisomerase
  • PGI
  • PHI
  • Phosphoglucose isomerase
  • phosphohexomutase
  • Phosphohexose isomerase
  • phosphosaccharomutase
  • SA-36
  • Sperm antigen 36
  • sperm antigen-36

Background

Glucose 6 phosphate isomerase belongs to the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways. The protein encoded by this gene is a dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. The protein functions in different capacities inside and outside the cell. In the cytoplasm, the gene product is involved in glycolysis and gluconeogenesis, while outside the cell it functions as a neurotrophic factor for spinal and sensory neurons. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Glucose 6 phosphate isomerase Antibody (NBP2-79845) (0)

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol GPI