Glucose 6 phosphate isomerase Antibody (CPTC-GPI-1) - Azide and BSA Free Summary
Description |
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-79711).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
Immunogen |
Recombinant human full-length Glucose 6 phosphate isomerase protein (Uniprot: P06744) |
Localization |
Cytoplasm (secreted) |
Isotype |
IgG2a Kappa |
Clonality |
Monoclonal |
Host |
Mouse |
Gene |
GPI |
Purity |
Protein A or G purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
- Immunohistochemistry 1-2 ug/ml
- Immunohistochemistry-Paraffin 1-2 ug/ml
- Protein Array
- Western Blot 1-2 ug/ml
|
Application Notes |
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
Theoretical MW |
55 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
Storage |
Store at -20 to -80C. Avoid freeze-thaw cycles. |
Buffer |
10 mM PBS |
Preservative |
No Preservative |
Concentration |
1 mg/ml |
Purity |
Protein A or G purified |
Alternate Names for Glucose 6 phosphate isomerase Antibody (CPTC-GPI-1) - Azide and BSA Free
Background
Glucose 6 phosphate isomerase belongs to the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways. The protein encoded by this gene is a dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. The protein functions in different capacities inside and outside the cell. In the cytoplasm, the gene product is involved in glycolysis and gluconeogenesis, while outside the cell it functions as a neurotrophic factor for spinal and sensory neurons. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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