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GIT2 Antibody (3B5-B9)

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Western Blot: GIT2 Antibody (3B5-B9) [H00009815-M01] - GIT2 monoclonal antibody (M01), clone 3B5-B9 Analysis of GIT2 expression in Jurkat.
Western Blot: GIT2 Antibody (3B5-B9) [H00009815-M01] - GIT2 monoclonal antibody (M01), clone 3B5-B9 Analysis of GIT2 expression in Jurkat.
Immunocytochemistry/ Immunofluorescence: GIT2 Antibody (3B5-B9) [H00009815-M01] - Analysis of monoclonal antibody to GIT2 on HeLa cell. Antibody concentration 20 ug/ml.
Immunohistochemistry-Paraffin: GIT2 Antibody (3B5-B9) [H00009815-M01] - Analysis of monoclonal antibody to GIT2 on formalin-fixed paraffin-embedded human lung. Antibody concentration 3 ug/ml.
Western Blot: GIT2 Antibody (3B5-B9) [H00009815-M01] - Analysis of GIT2 expression in transfected 293T cell line by GIT2 monoclonal antibody (M01), clone 3B5-B9.Lane 1: GIT2 transfected lysate(52.6 KDa).Lane 2: ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clone
3B5-B9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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GIT2 Antibody (3B5-B9) Summary

Immunogen
GIT2 (AAH01379, 1 a.a. ~ 471 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSKRLRSSEVCADCSGPDPSWASVNRGTFLCDECCSVHRSLGRHISQVRHLKHTPWPPTLLQMVETLYNNGANSIWEHSLLDPASIMSGRRKANPQDKVHPNKAEFIRAKYQMLAFVHRLPCRDDDSVTAKDLSKQLHSSVRTGNLETCLRLLSLGAQANFFHPEKGNTPLHVASKAGQILQAELLAVYGADPGTQDSSGKTPVDYARQGGHHELAERLVEIQYELTDRLAFYLCGRKPDHKNGQHFIIPQMADSSLDLSELAKAAKKKLQSLSNHLFEELAMDMYDEVDRRETDAVWLATQNHSALVTETTVVPFLPVNPEYSSTRNQGRQKLARFNAHEFATLVIDILSDAKRRQQGSSLSGSKDNVELILKTINNQHSVESQDNDQPDYDSVASDEDTDLETTASKTNRQKSLDSDLSDGPVTVQEFMEVKNALVASEAKIQQLMKVNNNLSDELRIMQKKLLGKDAN
Specificity
GIT2 - G protein-coupled receptor kinase interactor 2
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
GIT2
Purity
IgG purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for GIT2 Antibody (3B5-B9)

  • ARF GAP GIT2
  • ARF GTPase-activating protein GIT2
  • CAT2
  • CAT-2
  • cool-associated, tyrosine phosphorylated protein 2
  • Cool-interacting tyrosine-phosphorylated protein 2
  • DKFZp686G01261
  • G protein-coupled receptor kinase interacting ArfGAP 2
  • G protein-coupled receptor kinase interactor 2
  • G protein-coupled receptor kinase-interactor 2
  • KIAA0148GRK-interacting protein 2
  • MGC760

Background

This gene encodes a member of the GIT protein family. GIT proteins interact with G protein-coupled receptor kinases and possess ADP-ribosylation factor (ARF) GTPase-activating protein (GAP) activity. This gene undergoes extensive alternative splicing; although ten transcript variants have been described, the full length sequence has been determined for only four variants. The various isoforms have functional differences, with respect to ARF GAP activity and to G protein-coupled receptor kinase 2 binding.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol GIT2
Entrez
OMIM
Uniprot