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Gephyrin/GPHN Antibody [CoraFluor™ 1]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP (-)
Clonality
Polyclonal
Host
Rabbit
Conjugate
CoraFluor 1

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-34859AF350
Alexa Fluor 405
NBP2-34859AF405
Alexa Fluor 488
NBP2-34859AF488
Alexa Fluor 532
NBP2-34859AF532
Alexa Fluor 594
NBP2-34859AF594
Alexa Fluor 647
NBP2-34859AF647
Alexa Fluor 700
NBP2-34859AF700
Alexa Fluor 750
NBP2-34859AF750
Biotin
NBP2-34859B
DyLight 350
NBP2-34859UV
DyLight 405
NBP2-34859V
DyLight 488
NBP2-34859G
DyLight 550
NBP2-34859R
DyLight 594
NBP2-34859DL594
DyLight 650
NBP2-34859C
DyLight 680
NBP2-34859FR
DyLight 755
NBP2-34859IR
HRP
NBP2-34859H
Janelia Fluor 525
NBP2-34859JF525
Janelia Fluor 549
NBP2-34859JF549
Janelia Fluor 646
NBP2-34859JF646
Janelia Fluor 669
NBP2-34859JF669
Unconjugated
NBP2-34859
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Gephyrin/GPHN Antibody [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen
The immunogen this antibody was made to, maps to a region between residue 686 to 736 of human Gephyrin using the numbering given in entry NP_001019389.1 (GeneID 10243).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GPHN
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Immunogen affinity purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for Gephyrin/GPHN Antibody [CoraFluor™ 1]

  • GEPH
  • Gephyrin
  • GPH
  • GPHN
  • GPHRYN
  • KIAA1385GPHGEPH

Background

The Gephyrin (GPHN) gene encodes a neuronal assembly protein that exists in isoform 1 (736 amino acids long, 79 kDA) or isoform 2 (769 amino acids in length, approximately 83 kDA). This protein anchors inhibitory neurotransmitter receptors to the postsynaptic cytoskeleton through high affinity binding to a receptor subunit domain and tublin dimers. The protein coded by GPHN is also necessary for molybdenum biosynthesis. The GPHN gene has been investigated in relation to many diseases such as leukemia, cervicitis, tuberculosis, alzheimer's disease, anorexia nervosa, huntington's disease, retinitis, and herpes zoster. The GPHN gene interacts with genes DYNLL1, PFN1, PRPF4, ENAH, and SLX4 to participate in pathways that regulate metabolism of vitamins and cofactors as well as molybdenum cofactor biosynthesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol GPHN