![Western Blot: GBE1 Antibody (OTI1D11) - Azide and BSA Free [NBP2-70810] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GBE1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GBE1.](http://images.novusbio.com/fullsize/GBE1-Antibody-OTI1D11-Azide-and-BSA-Free-Western-Blot-NBP2-70810-img0002.jpg "Western Blot: GBE1 Antibody (OTI1D11) - Azide and BSA Free [NBP2-70810] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GBE1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GBE1.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, IP, CyTOF-ready |
| Clone | OTI1D11 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | LYOPH |
| Immunogen | Full length human recombinant protein of human GBE1 (NP_000149) produced in HEK293T cell. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | GBE1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 80.3 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from PBS (pH 7.3) with 8% Trehalose |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | Immunogen affinity purified |
| Reconstitution Instructions | we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | GBE1 |
![Immunocytochemistry/Immunofluorescence: GBE1 Antibody (OTI1D11) - Azide and BSA Free [NBP2-70810] - Staining of HepG2 cells using anti-GBE1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GBE1-Antibody-OTI1D11-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70810-img0004.jpg "Immunocytochemistry/Immunofluorescence: GBE1 Antibody (OTI1D11) - Azide and BSA Free [NBP2-70810] - Staining of HepG2 cells using anti-GBE1 mouse monoclonal antibody.")
![Immunohistochemistry: GBE1 Antibody (OTI1D11) - Azide and BSA Free [NBP2-70810] - Staining of paraffin-embedded Human Ovary tissue using anti-GBE1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GBE1-Antibody-OTI1D11-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70810-img0008.jpg "Immunohistochemistry: GBE1 Antibody (OTI1D11) - Azide and BSA Free [NBP2-70810] - Staining of paraffin-embedded Human Ovary tissue using anti-GBE1 mouse monoclonal antibody.")
![Flow Cytometry: GBE1 Antibody (OTI1D11) - Azide and BSA Free [NBP2-70810] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY GBE1.](http://images.novusbio.com/fullsize/GBE1-Antibody-OTI1D11-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70810-img0005.jpg "Flow Cytometry: GBE1 Antibody (OTI1D11) - Azide and BSA Free [NBP2-70810] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY GBE1.")
![Western Blot: GBE1 Antibody (OTI1D11) - Azide and BSA Free [NBP2-70810] - Analysis of extracts (35ug) from 9 different cell lines by using g anti-GBE1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/GBE1-Antibody-OTI1D11-Azide-and-BSA-Free-Western-Blot-NBP2-70810-img0001.jpg "Western Blot: GBE1 Antibody (OTI1D11) - Azide and BSA Free [NBP2-70810] - Analysis of extracts (35ug) from 9 different cell lines by using g anti-GBE1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
![Immunohistochemistry: GBE1 Antibody (OTI1D11) - Azide and BSA Free [NBP2-70810] - Staining of paraffin-embedded Human colon tissue using anti-GBE1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GBE1-Antibody-OTI1D11-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70810-img0006.jpg "Immunohistochemistry: GBE1 Antibody (OTI1D11) - Azide and BSA Free [NBP2-70810] - Staining of paraffin-embedded Human colon tissue using anti-GBE1 mouse monoclonal antibody.")
![Immunohistochemistry: GBE1 Antibody (OTI1D11) - Azide and BSA Free [NBP2-70810] - Staining of paraffin-embedded Human endometrium tissue using anti-GBE1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GBE1-Antibody-OTI1D11-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70810-img0007.jpg "Immunohistochemistry: GBE1 Antibody (OTI1D11) - Azide and BSA Free [NBP2-70810] - Staining of paraffin-embedded Human endometrium tissue using anti-GBE1 mouse monoclonal antibody.")
![Flow Cytometry: GBE1 Antibody (OTI1D11) - Azide and BSA Free [NBP2-70810] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-GBE1 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/GBE1-Antibody-OTI1D11-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70810-img0003.jpg "Flow Cytometry: GBE1 Antibody (OTI1D11) - Azide and BSA Free [NBP2-70810] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-GBE1 antibody, and then analyzed by flow cytometry.")
![Immunoprecipitation: GBE1 Antibody (OTI1D11) - Azide and BSA Free [NBP2-70810] - (Negative control: IP without adding anti-GBE1 antibody.). For each experiment, 500ul of DDK tagged GBE1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-GBE1 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.](http://images.novusbio.com/fullsize/GBE1-Antibody-OTI1D11-Azide-and-BSA-Free-Immunoprecipitation-NBP2-70810-img0009.jpg "Immunoprecipitation: GBE1 Antibody (OTI1D11) - Azide and BSA Free [NBP2-70810] - (Negative control: IP without adding anti-GBE1 antibody.). For each experiment, 500ul of DDK tagged GBE1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-GBE1 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.")
![HAI-2/SPINT2 Antibody [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "HAI-2/SPINT2 Antibody [Unconjugated]")
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")