![Western Blot: Gankyrin Antibody [NB100-1834] - Analysis of Gankyrin in HeLa cell lysate. ECL detection in 20 seconds.](http://images.novusbio.com/fullsize/Gankyrin-Antibody-Western-Blot-NB100-1834-img0003.jpg "Western Blot: Gankyrin Antibody [NB100-1834] - Analysis of Gankyrin in HeLa cell lysate. ECL detection in 20 seconds.")
| Reactivity | Hu, Mu, Rt, Po, Bv, ZeSpecies Glossary |
| Applications | WB, Flow, ICC/IF |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 0.97 mg/ml |
| Immunogen | A synthetic peptide made to an internal portion of the human Gankyrin protein sequence (between residues 100-200). [UniProt# O75832] |
| Predicted Species | Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%), Zebrafish (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | PSMD10 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This Gankyrin antibody is useful in Western blot, where a band is observed ~24 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Theoretical MW | 24 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Control |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | Tris-Glycine and 0.15M NaCl |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.97 mg/ml |
| Purity | Immunogen affinity purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Gankyrin Antibody (NB100-1834)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | PSMD10 |
![Immunocytochemistry/Immunofluorescence: Gankyrin Antibody [NB100-1834] - Gankryrin antibody (1:500) was tested in HepG2 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x.](http://images.novusbio.com/fullsize/Gankyrin-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1834-img0006.jpg "Immunocytochemistry/Immunofluorescence: Gankyrin Antibody [NB100-1834] - Gankryrin antibody (1:500) was tested in HepG2 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x.")
![Flow (Intracellular): Gankyrin Antibody [NB100-1834] - Intracellular staining of Jurkat cells (1 x 10^6 cells/ml) with Gankyrin antibody (orange) stained at a dilution of 1:500. Detected with a GtxRb Dylight 488 secondary. Shown with the secondary control (blue).](http://images.novusbio.com/fullsize/Gankyrin-Antibody-Flow-Intracellular-NB100-1834-img0009.jpg "Flow (Intracellular): Gankyrin Antibody [NB100-1834] - Intracellular staining of Jurkat cells (1 x 10^6 cells/ml) with Gankyrin antibody (orange) stained at a dilution of 1:500. Detected with a GtxRb Dylight 488 secondary. Shown with the secondary control (blue).")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")