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GAGE1 Antibody

Images

 
Immunohistochemistry-Paraffin: GAGE1 Antibody [NBP2-54704] - Staining of human colon shows no positivity in glandular cells as expected.
Immunohistochemistry-Paraffin: GAGE1 Antibody [NBP2-54704] - Staining of human testis shows strong nuclear positivity in subset of cells in seminiferious ducts.
Immunohistochemistry-Paraffin: GAGE1 Antibody [NBP2-54704] - Staining of human lymph node shows no positivity in non-germinal center cells as expected.
Immunohistochemistry-Paraffin: GAGE1 Antibody [NBP2-54704] - Staining of human cerebral cortex shows no positivity in neurons as expected.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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GAGE1 Antibody Summary

Immunogen
This antibody was developed against a Recombinant Protein corresponding to amino acids: PPEMIGPMRPEQFSDEVEPATPEEGEPATQRQDPAAA
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GAGE1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:5000 - 1:10000
  • Immunohistochemistry-Paraffin 1:5000 - 1:10000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
GAGE1 Recombinant Protein Antigen (NBP2-54704PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for GAGE1 Antibody

  • Antigen MZ2-F
  • Cancer/testis antigen 4.1
  • G antigen 1
  • GAGE-1
  • member 1
  • MGC33825
  • MZ2-F antigen

Background

GAGE1 belongs to a family of genes that are expressed in a variety of tumors but not in normal tissues, except for the testis. The sequences of the family members are highly related but differ by scattered nucleotide substitutions. The GAGE1 cDNA contains a 143-bp insertion, located in the coding sequence near the termination codon, that is absent from the other cDNAs.The antigenic peptide YRPRPRRY, which is also encoded by several other family members, is recognized by autologous cytolytic T lymphocytes. Nothing is presently known about the function of this protein. An alternatively spliced transcript variant has been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GAGE1