GAD1/GAD67 Antibody (GAD1/2563) [Janelia Fluor® 635]

Recombinant human GAD1/GAD67 protein fragment (around aa 72-135) (exact sequence is proprietary) (Uniprot: Q99259)

Cytoplasmic

GABAergic Neuronal Marker

This monoclonal antibody recognizes a protein of 67kDa, which is identified as glutamic acid decarboxylase 1 (GDA1). There are two forms of glutamic acid decarboxylases (GADs) that are found in the brain: GAD65 (also known as GAD2) and GAD67 (also known as GAD1. GAD65 and GAD67 are members of the group II decarboxylase family of proteins and are responsible for catalyzing the rate-limiting step in the production of GABA (-aminobutyric acid) from L-glutamic acid. Although both GADs are found in the brain, GAD65 localizes to synaptic vesicle membranes in nerve terminals, while GAD67 is distributed throughout the cell. GAD67 is responsible for the basal levels of GABA synthesis. In the case of a heightened demand for GABA in neurotransmission, GAD65 will transiently activate to assist in GABA production. The loss of GAD65 is detrimental and can impair GABA neurotransmission, however the loss of GAD67 is lethal.

IgG2b Kappa

Monoclonal

Mouse

GAD1

Protein A or G purified

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Optimal dilution of this antibody should be experimentally determined.