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FXYD5/Dysadherin Antibody

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FXYD5/Dysadherin was detected in immersion fixed paraffin-embedded sections of human stomach using Goat Anti-Human FXYD5/Dysadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8300) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes and cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
FXYD5/Dysadherin was detected in immersion fixed paraffin-embedded sections of human stomach using Goat Anti-Human FXYD5/Dysadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8300) at 1 µg/mL overnight ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF8300-SP
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FXYD5/Dysadherin Antibody Summary

Immunogen
E. coli-derived recombinant human FXYD5/Dysadherin
Gln22-Asp133
Accession # Q96DB9
Specificity
Detects human FXYD5/Dysadherin in direct ELISAs and Western blots. 
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
FXYD5
Purity Statement
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FXYD5/Dysadherin Antibody

  • DYSAD
  • Dysadherin
  • EF-8
  • FXYD domain containing ion transport regulator 5
  • FXYD domain-containing ion transport regulator 5
  • FXYD5
  • IWU1
  • KCT1
  • keratinocytes associated transmembrane protein 1
  • OIT2
  • PRO6241
  • RIC

Background

Human FXYD5 (FXYD domain-containing ion transport regulator 5 and Dysadherin) is a 24 kDa glycoprotein and a member of the FXYD family of single span type I membrane proteins. This group of proteins has been shown to interact with the Na,K-ATPase and to modulate its properties. FXYD5 is structurally different from other family members and has been suggested to play a role in regulating E-cadherin and promoting metastasis. FXYD5 is preferentially expressed in kidney, intestine, spleen, stomach and lung. Human FXYD5 is 30% aa identical to mouse Fxyd5.  

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FXYD5/Dysadherin
Antibody
AF8300
FXYD5/Dysadherin Antibody
Species: Hu
Applications: IHC

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Bioinformatics

Gene Symbol FXYD5
Uniprot