![Western Blot: EXOSC3 Antibody (5C3) [H00051010-M03] - Analysis of EXOSC3 expression in Hela S3 NE (Cat # L013V3).](http://images.novusbio.com/fullsize/EXOSC3-Antibody-5C3-Western-Blot-H00051010-M03-img0008.jpg "Western Blot: EXOSC3 Antibody (5C3) [H00051010-M03] - Analysis of EXOSC3 expression in Hela S3 NE (Cat # L013V3).")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, IHC, IP |
| Clone | 5C3 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | EXOSC3 (AAH02437, 1 a.a. ~ 275 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAEPASVAAESLAGSRARAARTVLGQVVLPGEELLLPEQEDAEGPGGAVERPLSLNARACSRVRVVCGPGLRRCGDRLLVTKCGRLRHKEPGSGSGGGVYWVDSQQKRYVPVKGDHVIGIVTAKSGDIFKVDVGGSEPASLSYLSFEGATKRNRPNVQVGDLIYGQFVVANKDMEPEMVCIDSCGRANGMGVIGQDGLLFKVTLGLIRKLLAPDCEIIQEVGKLYPLEIVFGMNGRIWVKAKTIQQTLILANILEACEHMTSDQRKQIFSRLAES |
| Specificity | EXOSC3 (5C3) |
| Isotype | IgG2a Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | EXOSC3 |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
Secondary Antibodies |
Isotype Controls |
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![Immunohistochemistry-Paraffin: EXOSC3 Antibody (5C3) [H00051010-M03] - Analysis of monoclonal antibody to EXOSC3 on formalin-fixed paraffin-embedded human kidney. Antibody concentration 3 ug/ml](http://images.novusbio.com/fullsize/EXOSC3-Antibody-5C3-Immunohistochemistry-Paraffin-H00051010-M03-img0005.jpg "Immunohistochemistry-Paraffin: EXOSC3 Antibody (5C3) [H00051010-M03] - Analysis of monoclonal antibody to EXOSC3 on formalin-fixed paraffin-embedded human kidney. Antibody concentration 3 ug/ml")
![Western Blot: EXOSC3 Antibody (5C3) [H00051010-M03] - Analysis of EXOSC3 expression in transfected 293T cell line by EXOSC3 monoclonal antibody (M03), clone 5C3. Lane 1: EXOSC3 transfected lysate (Predicted MW: 29.6 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/EXOSC3-Antibody-5C3-Western-Blot-H00051010-M03-img0009.jpg "Western Blot: EXOSC3 Antibody (5C3) [H00051010-M03] - Analysis of EXOSC3 expression in transfected 293T cell line by EXOSC3 monoclonal antibody (M03), clone 5C3. Lane 1: EXOSC3 transfected lysate (Predicted MW: 29.6 KDa). Lane 2: Non-transfected lysate.")
![Immunoprecipitation: EXOSC3 Antibody (5C3) [H00051010-M03] - Analysis of EXOSC3 transfected lysate using anti-EXOSC3 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with EXOSC3 monoclonal antibody.](http://images.novusbio.com/fullsize/EXOSC3-Antibody-5C3-Immunoprecipitation-H00051010-M03-img0006.jpg "Immunoprecipitation: EXOSC3 Antibody (5C3) [H00051010-M03] - Analysis of EXOSC3 transfected lysate using anti-EXOSC3 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with EXOSC3 monoclonal antibody.")
![Sandwich ELISA: EXOSC3 Antibody (5C3) [H00051010-M03] - Detection limit for recombinant GST tagged EXOSC3 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/EXOSC3-Antibody-5C3-Sandwich-ELISA-H00051010-M03-img0007.jpg "Sandwich ELISA: EXOSC3 Antibody (5C3) [H00051010-M03] - Detection limit for recombinant GST tagged EXOSC3 is approximately 0.03ng/ml as a capture antibody.")
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