![Western Blot: ER beta/NR3A2 Antibody [NB120-3577] - Western blot analysis was performed on nuclear enriched extracts (30 ug lysate) of LNCap (Lane 1), PC-3 (Lane 2), MCF-7 (Lane 3), MDA-MB-231 (Lane 4), T-47D (Lane 5) and HeLa (Lane 6). The blots were probed with an anti-rabbit polyclonal ER beta/NR3A2 antibody [NB120-3577] at 1 ug/mL and detected by chemiluminescence using an HRP conjugated goat anti-rabbit IgG (H+L) secondary antibody at 0.4 ug/mL. A 60 kDa band corresponding to Estrogen Receptor beta was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using a 10% Bis-Tris gel. Resolved proteins were then transferred onto a nitrocellulose membrane. The membrane was probed with the relevant primary and secondary antibody following blocking with 5% skim milk. Chemiluminescent detection was performed.](http://images.novusbio.com/fullsize/ER-beta-NR3A2-Antibody-Western-Blot-NB120-3577-img0005.jpg "Western Blot: ER beta/NR3A2 Antibody [NB120-3577] - Western blot analysis was performed on nuclear enriched extracts (30 ug lysate) of LNCap (Lane 1), PC-3 (Lane 2), MCF-7 (Lane 3), MDA-MB-231 (Lane 4), T-47D (Lane 5) and HeLa (Lane 6). The blots were probed with an anti-rabbit polyclonal ER beta/NR3A2 antibody [NB120-3577] at 1 ug/mL and detected by chemiluminescence using an HRP conjugated goat anti-rabbit IgG (H+L) secondary antibody at 0.4 ug/mL. A 60 kDa band corresponding to Estrogen Receptor beta was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using a 10% Bis-Tris gel. Resolved proteins were then transferred onto a nitrocellulose membrane. The membrane was probed with the relevant primary and secondary antibody following blocking with 5% skim milk. Chemiluminescent detection was performed.")
| Reactivity | Hu, Mu, Rt, Po, Bv, Ha, Pm, ShSpecies Glossary |
| Applications | WB, GS, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Synthetic peptide corresponding to residues A(55) E P Q K S P W C E A R S L E H(70) of rat ER beta. |
| Localization | Nuclear |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | ESR2 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | WB: Detects an approx. 55 - 62 kDa protein representing ER beta from rat brain homogenate. ChIP usage was reported in the scientific literature (PMID: 19897598). |
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| Theoretical MW | 59 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS with 1 mg/ml BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
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![Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody [NB120-3577] - Figure 1 illustrates immunolocalization of ER beta in primate amygdala using NB120-3577.](http://images.novusbio.com/fullsize/ER-beta-NR3A2-Antibody-Immunohistochemistry-Paraffin-NB120-3577-img0004.jpg "Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody [NB120-3577] - Figure 1 illustrates immunolocalization of ER beta in primate amygdala using NB120-3577.")
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