Western blot shows lysates of MCF-7 human breast cancer cell line, Saos-2 human osteosarcoma cell line, and T47D human breast cancer cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ER beta /NR3A2 ...read more
ER beta /NR3A2 was detected in immersion fixed LNCaP human prostate cancer cell line using Mouse Anti-Human ER beta /NR3A2 Monoclonal Antibody (Catalog # MAB7106) at 10 µg/mL for 3 hours at room temperature. Cells were ...read more
ER beta /NR3A2 was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human ER beta /NR3A2 Monoclonal Antibody (Catalog # MAB7106) at 10 µg/mL for 3 hours at room temperature. Cells were ...read more
MCF‑7 human breast cancer cell line was stained with Mouse Anti-Human ER beta /NR3A2 Monoclonal Antibody (Catalog # MAB7106, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed ...read more
Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line, T47D human breast cancer cell line, MDA‑MB‑231 human breast cancer cell line, and 3T3‑L1 mouse embryonic ...read more
ER beta/NR3A2 Antibody (733930) [Unconjugated] Summary
Immunogen
E. coli-derived recombinant human ER beta /NR3A2 Met1-Gly318 Accession # Q92731
Specificity
Detects human ER beta /NR3A2 in direct ELISAs and Western blot. In direct ELISA and Western blot, no cross-reactivity with recombinant human ER alpha is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ESR2
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for ER beta/NR3A2 Antibody (733930) [Unconjugated]
ER beta
Erb
ER-beta
ESR2
ESR-BETA
ESTRB
estrogen receptor 2 (ER beta)
estrogen receptor beta 4
estrogen receptor beta
NR3A2
NR3A2ESRB
Nuclear receptor subfamily 3 group A member 2
Background
Estrogen Receptor beta (ER beta ; NR3A2) is a member of the steroid receptor family. The natural ligand for ER is the classical estrogenic compound 17 beta -estradiol. ER beta is expressed in the granulosa cell layer of primary, secondary and mature follicles in the ovary, in bone, bladder, uterus, testis, epididymis, gastrointestinal tract, kidney, breast, heart, vessel wall, immune system, lung, pituitary, hippocampus and hypothalamus. Roles for ER beta in the reproductive and cardiovascular systems have been reported, although these are the subject of conflicting reports. ER beta has been postulated to act primarily as a modulator of ER alpha function. ER beta has been shown to form homodimers as well as heterodimers with ER alpha . Both ER alpha and ER beta can give rise to numerous isoforms.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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