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ER beta/NR3A2 Antibody (733930) [Unconjugated]

Images

 
Western blot shows lysates of MCF-7 human breast cancer cell line, Saos-2 human osteosarcoma cell line, and T47D human breast cancer cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ER beta /NR3A2 ...read more
ER beta /NR3A2 was detected in immersion fixed LNCaP human prostate cancer cell line using Mouse Anti-Human ER beta /NR3A2 Monoclonal Antibody (Catalog # MAB7106) at 10 µg/mL for 3 hours at room temperature. Cells were ...read more
ER beta /NR3A2 was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human ER beta /NR3A2 Monoclonal Antibody (Catalog # MAB7106) at 10 µg/mL for 3 hours at room temperature. Cells were ...read more
MCF‑7 human breast cancer cell line was stained with Mouse Anti-Human ER beta /NR3A2 Monoclonal Antibody (Catalog # MAB7106, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed ...read more
Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line, T47D human breast cancer cell line, MDA‑MB‑231 human breast cancer cell line, and 3T3‑L1 mouse embryonic ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, CyTOF-ready, Flow, ICC/IF
Clone
733930
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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ER beta/NR3A2 Antibody (733930) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human ER beta /NR3A2
Met1-Gly318
Accession # Q92731
Specificity
Detects human ER beta /NR3A2 in direct ELISAs and Western blot. In direct ELISA and Western blot, no cross-reactivity with recombinant human ER alpha is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ESR2
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Immunocytochemistry 8-25 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Simple Western 50 ug/mL
  • Western Blot 2 ug/mL
Publications
Read Publications using
MAB7106 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ER beta/NR3A2 Antibody (733930) [Unconjugated]

  • ER beta
  • Erb
  • ER-beta
  • ESR2
  • ESR-BETA
  • ESTRB
  • estrogen receptor 2 (ER beta)
  • estrogen receptor beta 4
  • estrogen receptor beta
  • NR3A2
  • NR3A2ESRB
  • Nuclear receptor subfamily 3 group A member 2

Background

Estrogen Receptor beta (ER beta ; NR3A2) is a member of the steroid receptor family. The natural ligand for ER is the classical estrogenic compound 17 beta -estradiol. ER beta is expressed in the granulosa cell layer of primary, secondary and mature follicles in the ovary, in bone, bladder, uterus, testis, epididymis, gastrointestinal tract, kidney, breast, heart, vessel wall, immune system, lung, pituitary, hippocampus and hypothalamus. Roles for ER beta in the reproductive and cardiovascular systems have been reported, although these are the subject of conflicting reports. ER beta has been postulated to act primarily as a modulator of ER alpha function. ER beta has been shown to form homodimers as well as heterodimers with ER alpha . Both ER alpha and ER beta can give rise to numerous isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ER beta/NR3A2 Antibody (MAB7106)(2)

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Video Protocols

WB Video Protocol

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Bioinformatics

Gene Symbol ESR2
Uniprot