Novus Biologicals products are now on bio-techne.com

Ephrin-B1 [p Tyr298] Antibody

Images

 
Western Blot: EphB1 [p Tyr298] Antibody [NB300-274] - Rat testes lysate showing specific immunolabeling of the ~46k EphriNBphosphorylated at Tyr298 (control, lane 1). The phosphospecificity of this labeling is shown in ...read more

Product Details

Summary
Reactivity Hu, Rt, Mu, Bv, Ca, Ch, Pm, Xp, ZeSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Ephrin-B1 [p Tyr298] Antibody Summary

Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ephrin-B1 conjugated to KLH. Accession # P28693
Modification
p Tyr298
Specificity
Specific for endogenous levels of the ~46 kDa Ephrin-B1 protein phosphorylated at Tyr298. Immunolabeling is completely eliminated by treatment with lambda-phosphatase.
Predicted Species
Mouse (100%), Zebrafish (100%), Bovine (100%), Xenopus (100%), Primate (100%), Chicken (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EFNB1
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
Application Notes
This antibody has been tested in Western blot on rat lysate, detecting a band ~46 kDa.
Theoretical MW
46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
Preservative
No Preservative
Purity
Antigen Affinity-purified

Alternate Names for Ephrin-B1 [p Tyr298] Antibody

  • Cek5-L
  • EFL3
  • EFL-3
  • EFNB1
  • ELK ligand
  • ELK-L
  • EphrinB1
  • Ephrin-B1
  • LERK-2
  • STRA-1

Background

EphrinB proteins are thought to play key roles in cellular functions as diverse as neuronal migration and blood vessel development. EphrinB molecules expressed at the membrane surface bind to the EphB family receptors on target cells during cell-to cell contact. This interaction leads to cell signaling in the target cell but also generates a reverse signal in the cell expressing EphrinB on its surface. This reverse signaling event is thought to be critical for vessel maturation and neuronal development. Importantly, tyrosine phosphorylation of EphrinB is thought to be a critical component of this reverse signaling event. Recent work suggests that phosphorylation of a specific EphrinB residue (Tyr298) plays a key role in EphrinB signaling. (3, 5)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF638
Species: Hu
Applications: CyTOF-ready, Flow, ICC, WB
AF496
Species: Hu, Mu, Rt
Applications: CyTOF-ready, Flow, ICC, WB
AF467
Species: Hu, Mu
Applications: CyTOF-ready, Flow, ICC, IHC, Simple Western, WB
1596-B1
Species: Rt
Applications: Bind
602-A1
Species: Mu
Applications: Bind
AF395
Species: Hu
Applications: IHC, WB
AF432
Species: Mu
Applications: CyTOF-ready, Flow, ICC, WB
NBP3-17510
Species: Hu
Applications: IHC, IHC-P
8415-A2
Species: Mu
Applications: BA
AF641
Species: Mu
Applications: IHC, WB
374-EA
Species: Hu
Applications: Bind
AF446
Species: Mu
Applications: CyTOF-ready, Flow, IHC, WB
AF3035
Species: Hu
Applications: CyTOF-ready, Flow, IHC, KO, WB
MAB369
Species: Hu
Applications: IHC, WB
NBP2-20486
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, IP, WB
NBP2-37364
Species: Hu, Mu
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-P, KD, WB
359-EA
Species: Hu
Applications: Bind
NB100-56603
Species: Ca, Hu, Mu, Pm, Rt
Applications: IHC, IHC-P, WB

Publications for Ephrin-B1 Antibody (NB300-274) (0)

There are no publications for Ephrin-B1 Antibody (NB300-274).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Ephrin-B1 Antibody (NB300-274) (0)

There are no reviews for Ephrin-B1 Antibody (NB300-274). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Ephrin-B1 Antibody (NB300-274) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Ephrin-B1 Products

Research Areas for Ephrin-B1 Antibody (NB300-274)

Find related products by research area.

Blogs on Ephrin-B1

There are no specific blogs for Ephrin-B1, but you can read our latest blog posts.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Ephrin-B1 [p Tyr298] Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol EFNB1
Uniprot