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ELK3 Antibody (OTI1H3) - Azide and BSA Free

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Western Blot: ELK3 Antibody (OTI1H3) - Azide and BSA Free [NBP2-70643] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ELK3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ELK3.
Western Blot: ELK3 Antibody (OTI1H3) - Azide and BSA Free [NBP2-70643] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ELK3 (Right lane) cDNA for 48 hrs and lysed. Equivalent ...read more
Immunocytochemistry/ Immunofluorescence: ELK3 Antibody (OTI1H3) - Azide and BSA Free [NBP2-70643] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ELK3.
Immunohistochemistry: ELK3 Antibody (OTI1H3) - Azide and BSA Free [NBP2-70643] - Staining of paraffin-embedded Human lymphoma tissue using anti-ELK3 mouse monoclonal antibody.
Flow Cytometry: ELK3 Antibody (OTI1H3) - Azide and BSA Free [NBP2-70643] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-ELK3 ...read more
Western Blot: ELK3 Antibody (OTI1H3) - Azide and BSA Free [NBP2-70643] - Breast cancer cells. Lane 1: MDA-MB231 + Negative control siRNA. Lane 2: MDA-MB231 + siELK3-1. Lane 3: MDA-MB231 + siELK3-2. Image from verified ...read more
Immunohistochemistry: ELK3 Antibody (OTI1H3) - Azide and BSA Free [NBP2-70643] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-ELK3 mouse monoclonal antibody.
Immunohistochemistry: ELK3 Antibody (OTI1H3) - Azide and BSA Free [NBP2-70643] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ELK3 mouse monoclonal antibody.
Immunohistochemistry: ELK3 Antibody (OTI1H3) - Azide and BSA Free [NBP2-70643] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-ELK3 mouse monoclonal antibody.
Immunohistochemistry: ELK3 Antibody (OTI1H3) - Azide and BSA Free [NBP2-70643] - Staining of paraffin-embedded Human tonsil using anti-ELK3 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, CyTOF-ready
Clone
OTI1H3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-70643AF350
Alexa Fluor 405
NBP2-70643AF405
Alexa Fluor 488
NBP2-70643AF488
Alexa Fluor 532
NBP2-70643AF532
Alexa Fluor 594
NBP2-70643AF594
Alexa Fluor 647
NBP2-70643AF647
Alexa Fluor 700
NBP2-70643AF700
Alexa Fluor 750
NBP2-70643AF750
Allophycocyanin
NBP2-70643APC
Biotin
NBP2-70643B
CoraFluor 1
NBP2-70643CL1
DyLight 350
NBP2-70643UV
DyLight 405
NBP2-70643V
DyLight 488
NBP2-70643G
DyLight 550
NBP2-70643R
DyLight 594
NBP2-70643DL594
DyLight 650
NBP2-70643C
DyLight 680
NBP2-70643FR
DyLight 755
NBP2-70643IR
FITC
NBP2-70643F
HRP
NBP2-70643H
Janelia Fluor 525
NBP2-70643JF525
Janelia Fluor 549
NBP2-70643JF549
Janelia Fluor 646
NBP2-70643JF646
Janelia Fluor 669
NBP2-70643JF669
PE
NBP2-70643PE
PerCP
NBP2-70643PCP
Unconjugated
NBP2-01264
mFluor Violet 450 SE
NBP2-70643MFV450
mFluor Violet 500 SE
NBP2-70643MFV500
mFluor Violet 610 SE
NBP2-70643MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-01264
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ELK3 Antibody (OTI1H3) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human ELK3(NP_005221) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ELK3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
Theoretical MW
44.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for ELK3 Antibody (OTI1H3) - Azide and BSA Free

  • ELK3, ETS-domain protein (SRF accessory protein 2)
  • ERPETS domain-containing protein Elk-3
  • ETS-domain protein
  • ETS-related protein ERP
  • NETSRF accessory protein 2
  • SAP-2
  • SAP2ETS-related protein NET
  • Serum response factor accessory protein 2

Background

ELK3 is encoded by this gene is a member of the ETS-domain transcription factor family and the ternary complex factor (TCF) subfamily. Proteins in this subfamily regulate transcription when recruited by serum response factor to bind to serum response elements. This protein is activated by signal-induced phosphorylation; studies in rodents suggest that it is a transcriptional inhibitor in the absence of Ras, but activates transcription when Ras is present. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol ELK3