EED Antibody [Unconjugated]

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

EED (Embryonic ectoderm development; also WAIT-1) is a 51 kDa member of the WD repeat ESC family of proteins. It is widely expressed, and constitutes part of both the PRC1 and PRC2 nuclear complexes. PRC1 contains EED, Suz12, RbAp46 and Ezh1 and is found in nonproliferating tissue. PRC2 differs only by the presence of Ezh2 and is found in proliferating tissues. Both complexes methylate histone H3 and act as gene repressors. Human EED is 441 amino acids in length. It contains three phosphorylation sites (Ser34; Thr55; Thr57) followed by seven WD40 domains (aa 91‑441). There are two alternate start sites, one at Met15, and another that is 94 aa upstream of the standard site, begins with a Val and generates a 69 kDa isoform. A third isoform variant shows an insertion of 25 aa after Ser323, while a fourth variant shows a premature truncation after Lys400. Human and mouse EED are identical in amino acid sequence.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: IHC-P, Simple Western, Western Blot.

Showing Publications 1 - 5 of 5.

Publications using AF5827	Applications	Species
VanDyke, D;Xu, L;Sargunas, PR;Gilbreth, RN;Baca, M;Gao, C;Hunt, J;Spangler, JB; Redirecting the specificity of tripartite motif containing-21 scaffolds using a novel discovery and design approach The Journal of biological chemistry 2023-10-20 [PMID: 37866632] (Western Blot, Human)	Western Blot	Human
Gabrielle J. Dardis, Jun Wang, Jeremy M. Simon, Gang Greg Wang, Albert S. Baldwin An EZH2-NF-kappa B regulatory axis drives expression of pro-oncogenic gene signatures in triple negative breast cancer iScience 2023-07-21 [PMID: 37416481]
Frances Potjewyd, Anne-Marie W. Turner, Joshua Beri, Justin M. Rectenwald, Jacqueline L. Norris-Drouin, Stephanie H. Cholensky et al. Degradation of Polycomb Repressive Complex 2 with an EED-Targeted Bivalent Chemical Degrader Cell Chemical Biology 2020-01-01 [PMID: 31831267] (Simple Western)	Simple Western
EM Michalak, MJG Milevskiy, RM Joyce, JF Dekkers, PR Jamieson, B Pal, CA Dawson, Y Hu, SH Orkin, WS Alexander, GJ Lindeman, GK Smyth, JE Visvader Canonical PRC2 function is essential for mammary gland development and affects chromatin compaction in mammary organoids PLoS Biol., 2018-08-06;16(8):e2004986. 2018-08-06 [PMID: 30080881] (IHC-P, Mouse)	IHC-P	Mouse
DJ Micati, GR Hime, EA McLaughlin, HE Abud, KL Loveland Differential expression profiles of conserved Snail transcription factors in the mouse testis Andrology, 2018-01-30;0(0):. 2018-01-30 [PMID: 29381885] (IHC-P, Mouse)	IHC-P	Mouse

Array AF5827

• EED Antibodies
• EED Lysates
• EED Proteins