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![Immunohistochemistry-Paraffin: EED Antibody [NBP2-38907] - Staining in human tonsil and pancreas tissues.. Corresponding EED RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/EED-Antibody-Immunohistochemistry-Paraffin-NBP2-38907-img0006.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: EED Antibody [NBP2-38907] - Staining in human tonsil and pancreas tissues.. Corresponding EED RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Western Blot: EED Antibody [NBP2-38907] - Analysis using Anti-EED antibody NBP2-38907 (A) shows similar pattern to independent antibody NBP2-57195 (B).](http://images.novusbio.com/fullsize/EED-Antibody-Western-Blot-NBP2-38907-img0010.jpg)
![Immunohistochemistry-Paraffin: EED Antibody [NBP2-38907] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/EED-Antibody-Immunohistochemistry-Paraffin-NBP2-38907-img0009.jpg)
![Immunohistochemistry-Paraffin: EED Antibody [NBP2-38907] - Staining of human tonsil shows strong nuclear positivity in germinal center cells.](http://images.novusbio.com/fullsize/EED-Antibody-Immunohistochemistry-Paraffin-NBP2-38907-img0005.jpg)
![Immunohistochemistry-Paraffin: EED Antibody [NBP2-38907] - Staining of human pancreas shows no positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/EED-Antibody-Immunohistochemistry-Paraffin-NBP2-38907-img0007.jpg)
![Immunohistochemistry-Paraffin: EED Antibody [NBP2-38907] - Staining of human rectum shows moderate nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/EED-Antibody-Immunohistochemistry-Paraffin-NBP2-38907-img0008.jpg)
![Western Blot: EED Antibody (GT885) [NBP3-13628] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with EED antibody [GT885] (NBP3-13628) diluted at 1:500. The HRP-conjugated anti-mouset IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/EED-Antibody-GT885-Western-Blot-NBP3-13628-img0001.jpg "Western Blot: EED Antibody (GT885) [NBP3-13628] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with EED antibody [GT885] (NBP3-13628) diluted at 1:500. The HRP-conjugated anti-mouset IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: EED Antibody (GT885) [NBP3-13628] - EED antibody [GT885] detects EED protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EED stained by EED antibody [GT885] (NBP3-13628) diluted at 1:1000. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/EED-Antibody-GT885-Immunocytochemistry-Immunofluorescence-NBP3-13628-img0004.jpg)
![Immunohistochemistry-Paraffin: EED Antibody (GT885) [NBP3-13628] - EED antibody [GT885] detects EED protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat endometrial carcinoma. EED stained by EED antibody [GT885] (NBP3-13628) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/EED-Antibody-GT885-Immunohistochemistry-Paraffin-NBP3-13628-img0003.jpg)
![Immunohistochemistry-Paraffin: EED Antibody (GT885) [NBP3-13628] - EED antibody [GT885] detects EED protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. EED stained by EED antibody [GT885] (NBP3-13628) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/EED-Antibody-GT885-Immunohistochemistry-Paraffin-NBP3-13628-img0002.jpg)
![Western Blot: EED Antibody [NBP2-81819] - Western blot analysis of EED expression in human testis tissue lysate with EED antibody at 2 ug/ml](http://images.novusbio.com/fullsize/EED-Antibody-Western-Blot-NBP2-81819-img0003.jpg "Western Blot: EED Antibody [NBP2-81819] - Western blot analysis of EED expression in human testis tissue lysate with EED antibody at 2 ug/ml")
![Western Blot: EED Antibody [NBP2-81819] - Analysis of EED in human heart tissue lysate with EED antibody at (A) 1 and (B) 2 ug/ml.](http://images.novusbio.com/fullsize/EED-Antibody-Western-Blot-NBP2-81819-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: EED Antibody - BSA Free [NBP2-81819] - Immunofluorescence of EED in human heart tissue with EED antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp2-81819_rabbit-polyclonal-eed-antibody-2711202419553024.jpg)
![Western Blot: EED Antibody [NBP2-57195] - Analysis using Anti-EED antibody NBP2-57195 (A) shows similar pattern to independent antibody NBP2-38907 (B).](http://images.novusbio.com/fullsize/EED-Antibody-Western-Blot-NBP2-57195-img0003.jpg "<b>Independent Antibodies Validation. </b>Western Blot: EED Antibody [NBP2-57195] - Analysis using Anti-EED antibody NBP2-57195 (A) shows similar pattern to independent antibody NBP2-38907 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: EED Antibody [NBP2-57195] - Staining of human cell line A-431 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/EED-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57195-img0001.jpg)
![Sandwich ELISA: EED Antibody (3B12) [H00008726-M05] - Detection limit for recombinant GST tagged EED is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/EED-Antibody-3B12-Sandwich-ELISA-H00008726-M05-img0001.jpg "Sandwich ELISA: EED Antibody (3B12) [H00008726-M05] - Detection limit for recombinant GST tagged EED is 0.1 ng/ml as a capture antibody.")
![SDS-Page: Recombinant Human EED Protein [NBP2-23020] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain](http://images.novusbio.com/fullsize/Recombinant-Human-EED-Protein-SDS-Page-NBP2-23020-img0001.jpg "SDS-Page: Recombinant Human EED Protein [NBP2-23020] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain")
![Western Blot: EED Overexpression Lysate (Adult Normal) [NBL1-10120] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for EED.](http://images.novusbio.com/fullsize/EED-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-10120-img0002.jpg "Western Blot: EED Overexpression Lysate (Adult Normal) [NBL1-10120] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for EED.")
![Western Blot: EED Overexpression Lysate (Adult Normal) [NBL1-10119] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for EED.](http://images.novusbio.com/fullsize/EED-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-10119-img0002.jpg "Western Blot: EED Overexpression Lysate (Adult Normal) [NBL1-10119] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for EED.")
![Western Blot: EED Antibody [NBP2-98765] - Lane A: HepG2 Whole Cell LysateLane B: NCI-H1299 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:45 kDaObserved band size:49 kDa](http://images.novusbio.com/fullsize/EED-Antibody-Western-Blot-NBP2-98765-img0001.jpg "Western Blot: EED Antibody [NBP2-98765] - Lane A: HepG2 Whole Cell LysateLane B: NCI-H1299 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:45 kDaObserved band size:49 kDa")