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EDNRA/Endothelin R Type A Antibody (485709) [Unconjugated]

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HEK293 human embryonic kidney cell line transfected with either (A) human EDNRA or (B) irrelevant protein and eGFP was stained with Mouse Anti-Human EDNRA Monoclonal Antibody (Catalog # MAB65381) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.
HEK293 human embryonic kidney cell line transfected with either (A) human EDNRA or (B) irrelevant protein and eGFP was stained with Mouse Anti-Human EDNRA Monoclonal Antibody (Catalog # MAB65381) followed by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, CyTOF-ready
Clone
485709
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB65381U-100UG
Alexa Fluor 405
FAB65381V-100UG
Alexa Fluor 488
FAB65381G-100UG
Alexa Fluor 532
FAB65381X
Alexa Fluor 594
FAB65381T-100UG
Alexa Fluor 647
FAB65381R-100UG
Alexa Fluor 700
FAB65381N-100UG
Alexa Fluor 750
FAB65381S-100UG
Allophycocyanin
FAB65381A
Allophycocyanin/Cy7
FAB65381APCCY7
Biotin
FAB65381B
CoraFluor 1
FAB65381CL1
DyLight 350
FAB65381D
DyLight 405
FAB65381E
DyLight 488
FAB65381K
DyLight 550
FAB65381L
DyLight 594
FAB65381M
DyLight 650
FAB65381W
DyLight 680
FAB65381Y
DyLight 755
FAB65381Z
FITC
FAB65381F
HRP
FAB65381H
Janelia Fluor 525
FAB65381JF525
Janelia Fluor 549
FAB65381I
Janelia Fluor 646
FAB65381J
Janelia Fluor 669
FAB65381JF669
PE
FAB65381P
PE/Atto594
FAB65381PEATT594
PE/Cy5.5
FAB65381PECY55
PE/Cy7
FAB65381PECY7
PerCP
FAB65381C
mFluor Violet 450 SE
FAB65381MFV450
mFluor Violet 500 SE
FAB65381MFV500
mFluor Violet 610 SE
FAB65381MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB65381-SP
Carrier Free
FAB65381S-100UG
Carrier Free
FAB65381R-100UG
Carrier Free
FAB65381N-100UG
Carrier Free
FAB65381G-100UG
Carrier Free
FAB65381V-100UG
Carrier Free
FAB65381U-100UG
Carrier Free
FAB65381T-100UG
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EDNRA/Endothelin R Type A Antibody (485709) [Unconjugated] Summary

Immunogen
NS0 mouse myeloma cell line transfected with human EDNRA/Endothelin R Type A
Accession # NP_001948
Specificity
Detects human EDNRA/Endothelin R Type A in direct ELISAs.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EDNRA/Endothelin R Type A Antibody (485709) [Unconjugated]

  • EDNRA
  • endothelin receptor type A
  • endothelin-1 receptor
  • endothelin-1-specific receptor
  • ET1-specific type
  • ETAR
  • ETRA
  • G protein-coupled receptor
  • hET-AR

Background

EDNRA (endothelin A receptor) is a 427 amino acid (aa) protein of the rhodopsin-like 7-transmembrane receptor family. It can be variably modified by N-linked glycoproteins, phosphorylation and palmitoylation.  It binds endothelins ET-1 and ET-2 preferentially, while EDNRB binds all three forms equally. Developmentally, EDNRA is expressed by cranial neural crest cells and induces facial morphogenesis. It is expressed by adult blood vessel smooth muscle cells, and is primarily responsible for the vasoconstrictor effects of ET-1. Human EDNRA shares 86% aa identity with mouse and rat EDNRA within the combined extracellular portions of the molecule.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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