![Western Blot: EB3 Antibody (KT36) [NBP3-23294] - Western blot of EB proteins in mouse brain (lanes 1-5). The blot was probed with rat monoclonals anti-EB1 (lane 1), anti-EB1/2/3 (lane 2), NBP3-23289 anti-EB2 (lane 3), NBP3-23293 anti-EB3 (lane 4), and NBP3-23294 anti-EB3 (lane 5). Then the antibodies were detected using goat anti-Rat IgG Light Chain specific: HRP.](http://images.novusbio.com/fullsize/nbp3-23294_rat-eb3-mab-kt36-11920231633169.jpg "Western Blot: EB3 Antibody (KT36) [NBP3-23294] - Western blot of EB proteins in mouse brain (lanes 1-5). The blot was probed with rat monoclonals anti-EB1 (lane 1), anti-EB1/2/3 (lane 2), NBP3-23289 anti-EB2 (lane 3), NBP3-23293 anti-EB3 (lane 4), and NBP3-23294 anti-EB3 (lane 5). Then the antibodies were detected using goat anti-Rat IgG Light Chain specific: HRP.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF |
| Clone | KT36 |
| Clonality | Monoclonal |
| Host | Rat |
| Conjugate | Unconjugated |
| Description | Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage). |
| Immunogen | Clone KT36 was generated from a GST fusion protein containing human EB3. This sequence is highly conserved in rat and mouse EB3. [UniProt# Q9UPY8] |
| Specificity | The antibody detects a 32 kDa protein corresponding to the molecular mass of EB3 on SDS-PAGE immunoblots of human HeLa cells and mouse brain tissue. The antibody also detects EB3 by immunocytochemistry in HeLa and A431 cells. |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Host | Rat |
| Gene | MAPRE3 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 32 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for EB3 Antibody (NBP3-23294)Find related products by research area.
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| Gene Symbol | MAPRE3 |
![Immunocytochemistry/Immunofluorescence: EB3 Antibody (KT36) [NBP3-23294] - Immunocytochemical labeling of EB3 in paraformaldehyde-fixed and NP40-permeabilized A431 cells. The cells were dual labeled with mouse monoclonal anti-alpha Tubulin (NBP3-23258) (green) and rat monoclonal anti-EB3 (NBP3-23294) (red). The antibodies were detected using either goat anti-mouse: DyLight(R) 488 or goat anti-Rat:DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23294_rat-eb3-mab-kt36-19920231856937.jpg "Immunocytochemistry/Immunofluorescence: EB3 Antibody (KT36) [NBP3-23294] - Immunocytochemical labeling of EB3 in paraformaldehyde-fixed and NP40-permeabilized A431 cells. The cells were dual labeled with mouse monoclonal anti-alpha Tubulin (NBP3-23258) (green) and rat monoclonal anti-EB3 (NBP3-23294) (red). The antibodies were detected using either goat anti-mouse: DyLight(R) 488 or goat anti-Rat:DyLight(R) 594.")
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