![Western Blot: DYKDDDDK Epitope Tag Antibody [NB600-345] - used for Western Blot. Lane 1: Untransfected HEK (control) Lane 2: Transfected HEK (FLAG-tagged protein, clone 1) Lane 3: Transfected HEK (FLAG-tagged protein, clone 2). Image from verified customer review.](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-Western-Blot-NB600-345-img0008.jpg "Western Blot: DYKDDDDK Epitope Tag Antibody [NB600-345] - used for Western Blot. Lane 1: Untransfected HEK (control) Lane 2: Transfected HEK (FLAG-tagged protein, clone 1) Lane 3: Transfected HEK (FLAG-tagged protein, clone 2). Image from verified customer review.")
| Reactivity | ETSpecies Glossary |
| Applications | WB, Simple Western, ELISA, ICC/IF, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Immunogen | To produce DYKDDDDK Epitope Tag Antibody, Rabbits were immunized with ECS enterokinase cleavage site (xxxDDDDK) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support. |
| Specificity | DYKDDDDK Epitope Tag Antibody reacts with FLAG. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Use in IHC reported in scientific literature (PMID: 33219296) ELISA for coating plates 1:100 - 1:500. Use in Western Blot reported in scientific literature (PMID: 30297740). In Simple Western internal validation: Abro1 NT Tx lysate at 0.1 mg/ml as sample; separated by size; antibody dilution of 1:250; matrix was 12-230 kDa; detected by Chemiluminescence. |
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| Theoretical MW | 1.01 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | PBS |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||||||
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reviewed by:
Lamberto Torralba |
IP | Human | 03/14/2022 |
Summary
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reviewed by:
Verified Customer |
WB | Human | 02/14/2022 |
Summary
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reviewed by:
Verified Customer |
ICC | Other | 08/03/2016 |
Summary
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reviewed by:
Verified Customer |
WB | Human | 08/08/2014 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for DYKDDDDK Epitope Tag Antibody (NB600-345)Find related products by research area.
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Developmental regulator Daam2 promotes glial cell tumors by degrading Von Hippel-Lindau protein By Jamshed Arslan Pharm.D. Glioblastoma is an aggressive type of cancer that forms from the star-shaped glial cells of the central nervous system, called astrocytes. Intriguingly, several genes linked to glioblasto... Read full blog post. |
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Hoist the DYKDDDDK Epitope Tag The DYKDDDDK Epitope Tag antibody was raised against a short, artificial sequence that is widely and commonly used as a protein fusion tag. It is pervasively present in a vast amount of scientific studies, and is a very powerful investigative research... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
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| Lamberto Torralba 03/14/2022 |
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| Application: | IP | |
| Species: | Human |
| Verified Customer 02/14/2022 |
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| Application: | WB | |
| Species: | Human |
| Verified Customer 08/03/2016 |
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| Application: | ICC | |
| Species: | Other |
![Immunocytochemistry/Immunofluorescence: DYKDDDDK Epitope Tag Antibody [NB600-345] - Samples: Human microvascular endothelial cells expressing FLAG tagged beta-catenin following transient transfection. Antibody: Affinity purified goat DYKDDDDK Epitope Tag Antibody [NB600-345] used at 1 ug/ml. Detection: FITC labeled rabbit anti-goat IgG (h+l).](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-Immunocytochemistry-Immunofluorescence-NB600-345-img0007.jpg "Immunocytochemistry/Immunofluorescence: DYKDDDDK Epitope Tag Antibody [NB600-345] - Samples: Human microvascular endothelial cells expressing FLAG tagged beta-catenin following transient transfection. Antibody: Affinity purified goat DYKDDDDK Epitope Tag Antibody [NB600-345] used at 1 ug/ml. Detection: FITC labeled rabbit anti-goat IgG (h+l).")
![Western Blot: DYKDDDDK Epitope Tag Antibody [NB600-345] - Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody: Affinity purified goat DYKDDDDK Epitope Tag Antibody [NB600-345] used for WB at 0.04 ug/ml (1:25,000). Detection: Chemiluminescence with an exposure time of 30 seconds. Observed molecular weight ~49 kDa](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-Western-Blot-NB600-345-img0002.jpg "Western Blot: DYKDDDDK Epitope Tag Antibody [NB600-345] - Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody: Affinity purified goat DYKDDDDK Epitope Tag Antibody [NB600-345] used for WB at 0.04 ug/ml (1:25,000). Detection: Chemiluminescence with an exposure time of 30 seconds. Observed molecular weight ~49 kDa")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
