| Reactivity | HuSpecies Glossary |
| Applications | WB, Simple Western, ICC/IF, KO |
| Clonality | Polyclonal |
| Host | Sheep |
| Conjugate | Unconjugated |
| Concentration | LYOPH |
| Immunogen | E. coli-derived recombinant human DNMT3B Ser145-Pro355 Accession # Q9UBC3 |
| Specificity | Detects human DNMT3B in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human DNMT3A is observed. |
| Source | N/A |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Sheep |
| Gene | DNMT3B |
| Purity Statement | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Publications |
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| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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| Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS. |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Reconstitution Instructions | Sterile PBS to a final concentration of 0.2 mg/mL. |
DNMT3B (DNA methyltransferase 3B; also DNA MTase HsaIIIB) is a 95-110 kDa member of the C5-methyltransferase family of enzymes. It is ubiquitously expressed in the embryo, and found associated with nuclear pericentric heterochromatin. Pericentric heterochromatin is chromatin composed of satellite repeats that lie adjacent to the centromere. DNA (cytosine-guanine dinucleotides) associated with this area undergos methylation, contributing to gene repression and proper chromosome segregation. Human DNMT3B is 853 amino acids (aa) in length. It contains a PWWP (Pro-Trp-Trp-Pro) domain that binds DNA (aa 225-283), a ADD (ATRX, DNMT3, DNMT3L) domain that contains a Zn-finger and binds to an unmethylated histone H3 tail (aa 423-555), and a class I AdoMet-MTase catalytic region that uses S-adenosyl-L-homocysteine as a substrate for methyl transfer (aa 577-847). There are at least five isoform variants. All show a deletion of aa 365-375. In addition, a second contains an alternative start site 12 aa upstream of the standard site, a third shows a deletion of aa 745-807, a fourth contains a Ser substitution for aa 744-853, while a fifth possesses a 45 aa substitution for aa 768-853. Over aa 1-355, human DNMT3B shares 73% aa sequence identity with mouse DNMT3B.
| Publication using AF7646 | Applications | Species |
|---|---|---|
| T Kuroda, S Yasuda, S Tachi, S Matsuyama, S Kusakawa, K Tano, T Miura, A Matsuyama, Y Sato SALL3 expression balance underlies lineage biases in human induced pluripotent stem cell differentiation Nat Commun, 2019-05-15;10(1):2175. 2019-05-15 [PMID: 31092818] (Immunoprecipitation, Western Blot, Human) | Immunoprecipitation, Western Blot | Human |
Secondary Antibodies |
Isotype Controls |
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Eat responsibly: Epigenetic downregulation of Ankrd26 gene by long-term high-fat intake promotes obesity and inflammation By Jamshed Arslan Pharm.D. White adipose tissue (WAT) represents the primary site to store energy in humans. WAT’s endocrine regulation of energy balance is controlled by nutritional status, exercise, and hormones l... Read full blog post. |
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The role of DNMT3B in the co-incidence of methyltransferase and tumor suppressor expression in malignancies Epigenetics is the process of heritable change in gene activity despite alteration of the hosts DNA sequence, essentially causing a change in a phenotype without a change in the genotype of a host. To change the gene sequence without interfering w... Read full blog post. |
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The role of DNMT3A in development Epigenetics is the study of heritable change in gene activity despite alteration of the hosts DNA sequence. Change in gene activity done independently of the DNA sequence is achieved by way of histone and DNA methylation. Gene silencing in DNA ... Read full blog post. |
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Go Ahead! Make My DNA DNA methylation plays a critical role the long-term silencing of transcription and is essential for processes such as embryonic development, germline differentiation, and tissue maturation. Dnmt3a is a member of the C5-methyltransferase family that re... Read full blog post. |
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Controlling Epigenetic Signaling with Dnmt1 and Dnmt3b Dnmt1 belongs to the C5-methyltransferase family that repairs cytosines in dsDNA using a nucleophilic attack mechanism. Dnmt1 is the most abundant mammalian DNA methyltransferase. It is the key methylation maintenance enzyme for both DNA replication/r... Read full blog post. |
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DNMT3B: Roles in Leukemia DNA-methyltransferase 3B (DNMT3B), also known as DNA methyltransferase HsaIIIB, is a member of the class I-like SAM-binding methyltransferase superfamily and C5-methyltransferase family. DNMT3B plays an essential role in the establishment of DNA methy... Read full blog post. |
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Not as Pluripotent as You Used to Be: Embryonic Stem Cell Markers and the Aging Process We at Novus Biologicals recently extended our antibody catalog to include several embryonic stem cells (ESC) antibodies validated for use in FACS (fluorescent activated cell sorting) assays. Among them was Oct4, which recently became the focus of an i... Read full blog post. |
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