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DMAP1 Antibody

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Biological Strategies: Western Blot: DMAP1 Antibody [NB100-256] - Detection of Human DMAP1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. ...read more
Western Blot: DMAP1 Antibody [NB100-256] - Detection of Human DMAP1 by Western Blot. Sample: Whole cell extract (50 mcg/lane) from HeLa cells. Antibody: Affinity purified goat anti-DMAP1 (Cat. No. NB100-256) used at ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

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DMAP1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 400 and 450 of human DNA Methyltransferase 1 Associated Protein 1 using the numbering given in entry NP_061973.1 (GeneID 55929).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
DMAP1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:5000
Application Notes
By Western blot, this antibody recognizes bands around 60 kDa representing DMAP1.
Theoretical MW
53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
HeLa Whole Cell Lysate
DMAP1 Overexpression Lysate

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DMAP1 Antibody

  • DNA methyltransferase 1 associated protein 1
  • DNA methyltransferase 1-associated protein 1
  • DNMAP1DNMT1-associated protein 1
  • DNMT1 associated protein 1
  • DNMTAP1
  • EAF2
  • FLJ11543
  • KIAA1425DKFZp686L09142
  • MEAF2
  • SWC4

Background

DMAP1 (DNA methyltransferase 1 associated protein 1) has been identified as a subunit of several distinct protein complexes involved in the repression and activation of transcription. It has been observed to interact with histone deacetylase 2 (HDAC2) as well as histone acetyltransferase (HAT) indicating a role in nucleosome modification to regulate transcription. DMAP1 can act independently to repress transcription in association with DNA methyltransferase 1. Alternate names for DMAP1 include DNMT1-associated protein 1, DNMAP1, DMAP1, KIAA1425, EAF2, SWC4, DNMAP1, DNMTAP1, FLJ11543, and DKFZp686L09142.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol DMAP1
Entrez
Uniprot