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DGK-zeta Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: DGK-zeta Antibody [NBP2-13918] - Staining of human cell line A-431 shows localization to nuclear speckles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: DGK-zeta Antibody [NBP2-13918] - Staining of human liver shows no positivity in hepatocytes.
Immunohistochemistry-Paraffin: DGK-zeta Antibody [NBP2-13918] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.
Immunohistochemistry-Paraffin: DGK-zeta Antibody [NBP2-13918] - Staining of human lymph node shows moderate cytoplasmic positivity in non - germinal center cells.
Immunohistochemistry-Paraffin: DGK-zeta Antibody [NBP2-13918] - Staining of human cerebral cortex shows weak cytoplasmic positivity in neurons.
Immunohistochemistry-Paraffin: DGK-zeta Antibody [NBP2-13918] - Staining of human small intestine shows weak cytoplasmic positivity in lymphoid tissue.
Western Blot-DGK-zeta Antibody-NBP2-13918-Analysis in human cell line SCLC-21H.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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DGK-zeta Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to the amino acids: EHLNYVTEIAQDEIYILDPELLGASARPDLPTPTSPLPTSPCSPTPRSLQGDAAPPQGEELIEAAKRNDFCKLQE
Predicted Species
Mouse (92%), Rat (96%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DGKZ
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF Fixation/Permeabilization: PFA/Triton X-100.
Control Peptide
DGK-zeta Protein (NBP2-13918PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for DGK-zeta Antibody

  • DAGK5
  • DAGK6DAG kinase zeta
  • DGKZ
  • DGKzeta
  • DGK-zeta
  • diacylglycerol kinase zeta
  • diacylglycerol kinase, zeta 104kDa
  • diacylglycerol kinase, zeta
  • Diglyceride kinase zeta
  • EC 2.7.1.107
  • hDGKzeta

Background

DGKZ, also known as Diacylglycerol kinase zeta, consists of six isoforms of sizes 124.1 kDa, 104 kDa, 106 kDa, 104.1 kDa, 104.6 kDa, and 101.2 kDa and is involved in regulating the amount of diacylglycerol in signal transduction pathways as a member of the eukaryotic diacylglycerol kinase family. Current research is exploring the effect of the protein on diseases such as malaria, retinoblastoma, cerebritis, bipolar disorder, and cerebral infarction. The protein interacts with RB1, RBL1, RBL2, MAPK6, and GCOM1 while in glycerolipid metabolism, hemostasis, PIP2 hydrolysis, and signal transduction pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol DGKZ