![Immunohistochemistry-Paraffin: Desmoglein-3 Antibody (DSG3/2839) - Azide and BSA Free [NBP3-08480] - Formalin-fixed, paraffin-embedded human Esophageal Carcinoma stained with Desmoglein-3 Mouse Monoclonal Antibody (DSG3/2839).](http://images.novusbio.com/fullsize/Desmoglein-3-Antibody-DSG3-2839-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-08480-img0002.jpg "Immunohistochemistry-Paraffin: Desmoglein-3 Antibody (DSG3/2839) - Azide and BSA Free [NBP3-08480] - Formalin-fixed, paraffin-embedded human Esophageal Carcinoma stained with Desmoglein-3 Mouse Monoclonal Antibody (DSG3/2839).")
| Reactivity | HuSpecies Glossary |
| Applications | IHC, MA |
| Clone | DSG3/2839 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07885). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | Recombinant fragment (around aa 379-491) human Desmoglein-3 protein (exact sequence is proprietary) (Uniprot: P32926) |
| Localization | Cell Surface |
| Marker | Squamous Cell Marker |
| Specificity | Recognizes a protein of 130kDa, identified as Desmoglein-3 (DSG3). This monoclonal antibody is highly specific to Desmoglein-3 and does not cross-react with other members of the Desmoglein-family. DSG3 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Research has shown that DSG3 has a very high sensitivity (80%) and specificity (100%) in recognizing squamous cell carcinoma (SqCC). Therefore, DSG3 is considered a very important marker for lung SqCC and can be a useful ancillary marker to separate SqCC from other subtypes of lung cancer. Moreover, studies have shown that DSG3 expression in lung SqCC may indicate a poor prognosis. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | DSG3 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. |
| Theoretical MW | 130 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein A or G purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Desmoglein-3 Antibody (NBP3-08480)Find related products by research area.
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Epithelial-Mesenchymal Transition (EMT) Markers Epithelial-Mesenchymal Transition (EMT) is the trans-differentiation of stationary epithelial cells into motile mesenchymal cells. During EMT, epithelial cells lose their junctions and apical-basal polarity, reorganize their cytoskeleton, undergo a... Read full blog post. |
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| Gene Symbol | DSG3 |
![Immunohistochemistry-Paraffin: Desmoglein-3 Antibody (DSG3/2839) - Azide and BSA Free [NBP3-08480] - Formalin-fixed, paraffin-embedded human Esophageal Carcinoma stained with Desmoglein-3 Mouse Monoclonal Antibody (DSG3/2839).](http://images.novusbio.com/fullsize/Desmoglein-3-Antibody-DSG3-2839-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-08480-img0001.jpg "Immunohistochemistry-Paraffin: Desmoglein-3 Antibody (DSG3/2839) - Azide and BSA Free [NBP3-08480] - Formalin-fixed, paraffin-embedded human Esophageal Carcinoma stained with Desmoglein-3 Mouse Monoclonal Antibody (DSG3/2839).")
![Immunohistochemistry-Paraffin: Desmoglein-3 Antibody (DSG3/2839) - Azide and BSA Free [NBP3-08480] - Formalin-fixed, paraffin-embedded human Esophageal Carcinoma stained with Desmoglein-3 Mouse Monoclonal Antibody (DSG3/2839).](http://images.novusbio.com/fullsize/Desmoglein-3-Antibody-DSG3-2839-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-08480-img0003.jpg "Immunohistochemistry-Paraffin: Desmoglein-3 Antibody (DSG3/2839) - Azide and BSA Free [NBP3-08480] - Formalin-fixed, paraffin-embedded human Esophageal Carcinoma stained with Desmoglein-3 Mouse Monoclonal Antibody (DSG3/2839).")
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