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Cytosolic Sulfotransferase 4A1/SULT4A1 Antibody (634630)

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Western blot shows lysates of human brain (cortex) tissue. PVDF Membrane was probed with 2 µg/mL of Human Cytosolic Sulfotransferase 4A1/SULT4A1 Monoclonal Antibody (Catalog # MAB5826) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Cytosolic Sulfotransferase 4A1/SULT4A1 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of human brain (cortex) tissue. PVDF Membrane was probed with 2 µg/mL of Human Cytosolic Sulfotransferase 4A1/SULT4A1 Monoclonal Antibody (Catalog # MAB5826) followed by HRP-conjugated ...read more
Cytosolic Sulfotransferase 4A1/SULT4A1 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Human Cytosolic Sulfotransferase 4A1/SULT4A1 Monoclonal Antibody (Catalog # MAB5826) at 15 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
634630
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB5826-SP
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Cytosolic Sulfotransferase 4A1/SULT4A1 Antibody (634630) Summary

Immunogen
E. coli-derived recombinant human Cytosolic Sulfotransferase 4A1/SULT4A1
Ala2-Lys284
Accession # Q9BR01
Specificity
Detects human Cytosolic Sulfotransferase 4A1/SULT4A1 in direct ELISAs and Western blots. No cross-reactivity with recombinant human (rh) SULT1A1, rhSULT1A3, rhSULT1B1, rhSULT1E1, rhSULT2A1, or rhSULT2B1 is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
SULT4A1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cytosolic Sulfotransferase 4A1/SULT4A1 Antibody (634630)

  • Brain sulfotransferase-like protein
  • BRSTL1
  • BR-STL-1
  • Cytosolic Sulfotransferase 4A1
  • EC 2.8.2.-
  • hBR-STL
  • hBR-STL-1MGC40032
  • nervous system cytosolic sulfotransferase
  • Nervous system sulfotransferase
  • NST
  • ST4A1
  • sulfotransferase 4A1
  • sulfotransferase family 4A, member 1
  • sulfotransferase-related protein
  • SULT4A1
  • SULTX3
  • SULTX3DJ388M5.3

Background

Cytosolic SULT4A1 (Sulfotransferase 4A1 also BR-STL1/Brain sulfotransferase-like protein) is a 33-36 kDa member of the sulfotransferase 4 family of enzymes.  SULT4A1 message is widely expressed.  However, it appears to be translated only in brain.  SULT4A is very unusual in that it is the only SULT that shows minimal activity on synthetic laboratory substrates.  Instead, it acts on catecholamines and T4 in a manner that may not involve sulfonation.  Human SULT4A1 is 284 amino acids (aa) in length.  A weak PAPS binding site is found between aa 246-254.  There are two potential isoforms, one that shows a 13 aa substitution for aa 248-284, and a second that contains a 48 aa substitution for aa 202-247.  Full length human SULT4A1 is 98% aa identical to mouse SULT4A1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Cytosolic
Sulfotransferase 4A1/ ...
MAB5826
Cytosolic Sulfotransferase 4A1/SULT4A1 Antibody (634630)
Species: Hu
Applications: WB, IHC

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Bioinformatics

Gene Symbol SULT4A1
Uniprot