Reactivity | HuSpecies Glossary |
Applications | IHC |
Clone | rKRTH/8883 |
Clonality | Monoclonal |
Host | Mouse |
Conjugate | Unconjugated |
Concentration | 0.2 mg/ml |
Description | Store at 2 to 8C. Non-hazardous. No MSDS required. |
Additional Information | Recombinant Monoclonal Antibody |
Immunogen | Recombinant full-length human Cytokeratin, HMW protein |
Localization | Cytoplasm. |
Marker | Epithelial Marker |
Specificity | This monoclonal antibody recognizes basic (Type II or HMW) cytokeratins, which include 67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8). Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50 , 48, 46, 45, and 40kDa. monoclonal antibody AE3 recognizes the 65-67, 64, 59, 58, 56, and 52kDa keratins of basic subfamily. |
Isotype | IgG2b Kappa |
Clonality | Monoclonal |
Host | Mouse |
Gene | KRT76 |
Purity | Protein A or G purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
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Application Notes | Immunohistochemistry-Paraffin: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
Storage | Store at 4C. |
Buffer | 10mM PBS with 0.05% BSA |
Preservative | 0.05% Sodium Azide |
Concentration | 0.2 mg/ml |
Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
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Gene Symbol | KRT76 |