![Western Blot: Cytokeratin, HMW Antibody (34BE12) - Azide and BSA Free [NBP2-34533] - Western Blot Analysis of MCF-7 cell lysate using Cytokeratin, HMW Antibody (34BE12).](http://images.novusbio.com/fullsize/Cytokeratin-HMW-Antibody-34BE12-Azide-and-BSA-Free-Western-Blot-NBP2-34533-img0002.jpg "Western Blot: Cytokeratin, HMW Antibody (34BE12) - Azide and BSA Free [NBP2-34533] - Western Blot Analysis of MCF-7 cell lysate using Cytokeratin, HMW Antibody (34BE12).")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, CyTOF-ready |
| Clone | 34BE12 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1.0 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-34340). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | Solubilized keratin extract from human stratum corneum |
| Localization | Cytoplasmic |
| Marker | Epithelial Marker |
| Specificity | This antibody recognizes CK1, CK5, CK10 and CK14. In normal epithelia, it stains stratified epithelia, myoepithelial cells and basal cells in the prostate gland and bronchi. This monoclonal antibody shows no reactivity with hepatocytes, pancreatic acinar cells, proximal renal tubules, or endometrial glands; there is no reactivity with cells derived from simple epithelia. Mesenchymal tumors, lymphomas, melanomas, neural tumors, and neuroendocrine tumors are negative with this antibody. It stains myoepithelial cells and has been shown to be useful in distinguishing prostate adenocarcinoma from benign prostate. This antibody has also been useful in separating benign from malignant intraductal breast proliferations. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | KRT76 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Application Notes | Immunohistochemistry (Formalin-fixed): 0.25-0.5ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1.0 mg/ml |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | KRT76 |
![Immunocytochemistry/Immunofluorescence: Cytokeratin, HMW Antibody (34BE12) - Azide and BSA Free [NBP2-34533] - Immunofluorescence Analysis of MeOH-fixed HeLa cells labeling Cytokeratin with followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot.](http://images.novusbio.com/fullsize/Cytokeratin-HMW-Antibody-34BE12-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-34533-img0001.jpg "Immunocytochemistry/Immunofluorescence: Cytokeratin, HMW Antibody (34BE12) - Azide and BSA Free [NBP2-34533] - Immunofluorescence Analysis of MeOH-fixed HeLa cells labeling Cytokeratin with followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot.")
![Immunohistochemistry-Paraffin: Cytokeratin, HMW Antibody (34BE12) - Azide and BSA Free [NBP2-34533] - Formalin-fixed, paraffin-embedded human prostate (20X) stained with Cytokeratin, HMW MAb (34bE12).](http://images.novusbio.com/fullsize/Cytokeratin-HMW-Antibody-34BE12-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34533-img0003.jpg "Immunohistochemistry-Paraffin: Cytokeratin, HMW Antibody (34BE12) - Azide and BSA Free [NBP2-34533] - Formalin-fixed, paraffin-embedded human prostate (20X) stained with Cytokeratin, HMW MAb (34bE12).")
![Immunohistochemistry-Paraffin: Cytokeratin, HMW Antibody (34BE12) - Azide and BSA Free [NBP2-34533] - Formalin-fixed, paraffin-embedded human prostate (10X) stained with Cytokeratin, HMW MAb (34bE12).](http://images.novusbio.com/fullsize/Cytokeratin-HMW-Antibody-34BE12-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34533-img0004.jpg "Immunohistochemistry-Paraffin: Cytokeratin, HMW Antibody (34BE12) - Azide and BSA Free [NBP2-34533] - Formalin-fixed, paraffin-embedded human prostate (10X) stained with Cytokeratin, HMW MAb (34bE12).")
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![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")