![Immunocytochemistry/Immunofluorescence: Cytokeratin 7 Antibody (KRT7/903) - Azide and BSA Free [NBP3-11426] - Immunofluorescence Analysis of methanol-fixed HeLa cells. Cytokeratin 7 antibody (KRT7/903)followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Redot.](http://images.novusbio.com/fullsize/Cytokeratin-7-Antibody-KRT7-903-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP3-11426-img0001.jpg "Immunocytochemistry/Immunofluorescence: Cytokeratin 7 Antibody (KRT7/903) - Azide and BSA Free [NBP3-11426] - Immunofluorescence Analysis of methanol-fixed HeLa cells. Cytokeratin 7 antibody (KRT7/903)followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Redot.")
| Reactivity | Hu, Ma(-), Mu(-), Rb(-), Rt(-)Species Glossary |
| Applications | Flow, ICC/IF, IHC |
| Clone | KRT7/903 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-44817). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous. |
| Immunogen | Recombinant full-length human Cytokeratin 7 protein (Uniprot: P08729) |
| Localization | Cytoplasmic |
| Marker | Glandular and Transitional Epithelial Marker |
| Specificity | Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. Antibody to cytokeratin is useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-). |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | KRT7 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris Buffer with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Theoretical MW | 54 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein A or G purified |
![Choline Kinase beta [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Choline Kinase beta [Unconjugated]")
Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | KRT7 |
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![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")