![Western Blot: Cytokeratin 7 Antibody (K72.7) - Azide and BSA Free [NBP2-47941] - Human HeLa cell lysate using Cytokeratin 7 Mouse Monoclonal Antibody (K72.7).](http://images.novusbio.com/fullsize/Cytokeratin-7-Antibody-K72-7-Azide-and-BSA-Free-Western-Blot-NBP2-47941-img0005.jpg "Western Blot: Cytokeratin 7 Antibody (K72.7) - Azide and BSA Free [NBP2-47941] - Human HeLa cell lysate using Cytokeratin 7 Mouse Monoclonal Antibody (K72.7).")
| Reactivity | HuSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, CyTOF-ready, ICC/IF |
| Clone | K72.7 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1.0 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-44816). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | Semi-Purified cytokeratin preparation. |
| Localization | Cytoplasmic |
| Marker | Glandular and Transitional Epithelial Marker |
| Specificity | It recognizes an intermediate filament protein (IFP) of 55kDa, which is identified as cytokeratin 7. This monoclonal antibody is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This monoclonal antibody is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-). |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | KRT7 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Theoretical MW | 55 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1.0 mg/ml |
| Purity | Protein A or G purified |
![Choline Kinase beta [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Choline Kinase beta [Unconjugated]")
Secondary Antibodies |
Isotype Controls |
Research Areas for Cytokeratin 7 Antibody (NBP2-47941)Find related products by research area.
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| Gene Symbol | KRT7 |
![Immunocytochemistry/Immunofluorescence: Cytokeratin 7 Antibody (K72.7) - Azide and BSA Free [NBP2-47941] - MeOH-fixed HeLa cells labeling KRT7 with KRT7 Mouse Monoclonal Antibody (K72.7) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Redot.](http://images.novusbio.com/fullsize/Cytokeratin-7-Antibody-K72-7-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-47941-img0004.jpg "Immunocytochemistry/Immunofluorescence: Cytokeratin 7 Antibody (K72.7) - Azide and BSA Free [NBP2-47941] - MeOH-fixed HeLa cells labeling KRT7 with KRT7 Mouse Monoclonal Antibody (K72.7) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Redot.")
![Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (K72.7) - Azide and BSA Free [NBP2-47941] - Ovarian Carcinoma stained with Cytokeratin 7 Mouse Monoclonal Antibody (K72.7).](http://images.novusbio.com/fullsize/Cytokeratin-7-Antibody-K72-7-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47941-img0002.jpg "Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (K72.7) - Azide and BSA Free [NBP2-47941] - Ovarian Carcinoma stained with Cytokeratin 7 Mouse Monoclonal Antibody (K72.7).")
![Flow Cytometry: Cytokeratin 7 Antibody (K72.7) - Azide and BSA Free [NBP2-47941] - PFA-fixed HeLa cells using KRT7 Mouse Monoclonal Antibody (K72.7) followed by Goat anti-Mouse IgG-CF488 (blue); Isotype Control (red).](http://images.novusbio.com/fullsize/Cytokeratin-7-Antibody-K72-7-Azide-and-BSA-Free-Flow-Cytometry-NBP2-47941-img0003.jpg "Flow Cytometry: Cytokeratin 7 Antibody (K72.7) - Azide and BSA Free [NBP2-47941] - PFA-fixed HeLa cells using KRT7 Mouse Monoclonal Antibody (K72.7) followed by Goat anti-Mouse IgG-CF488 (blue); Isotype Control (red).")
![Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (K72.7) - Azide and BSA Free [NBP2-47941] - Human Lung SCC stained with Cytokeratin 7 Mouse Monoclonal Antibody (K72.7).](http://images.novusbio.com/fullsize/Cytokeratin-7-Antibody-K72-7-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47941-img0001.jpg "Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (K72.7) - Azide and BSA Free [NBP2-47941] - Human Lung SCC stained with Cytokeratin 7 Mouse Monoclonal Antibody (K72.7).")
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![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")