![Cyclin B1 Antibody (SPM619) [mFluor Violet 610 SE] [NBP3-11598MFV610] - Vial of mFluor Violet 610 conjugated antibody. mFluor Violet 610 is optimally excited at 421 nm by the Violet laser (405 nm) and has an emission maximum of 613 nm.](http://images.novusbio.com/fullsize/nbp3-11598mfv610_mouse-cyclin-b1-mab-spm619-mfluor-violet-610-se-672023943037.png "Cyclin B1 Antibody (SPM619) [mFluor Violet 610 SE] [NBP3-11598MFV610] - Vial of mFluor Violet 610 conjugated antibody. mFluor Violet 610 is optimally excited at 421 nm by the Violet laser (405 nm) and has an emission maximum of 613 nm.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | Flow, ICC/IF, IHC, ICC/IF |
| Clone | SPM619 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | mFluor Violet 610 SE |
| Immunogen | Recombinant human full-length Cyclin B1 protein (Uniprot: P14635) |
| Localization | Cytoplasmic & Nuclear |
| Specificity | It recognizes a protein of 55-62kDa, identified as cyclin B1. In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CCNB1 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Optimal dilution of this antibody should be experimentally determined. |
| Storage | Store at 4C in the dark. |
| Buffer | 50mM Sodium Borate |
| Preservative | 0.05% Sodium Azide |
| Purity | Protein A or G purified |
![Western Blot PAK3 Antibody [Unconjugated]](https://images.novusbio.com/images2/PAK3_AF6897_Western_Blot_11082.jpg)
![Immunohistochemistry PAK3 Antibody [Unconjugated]](https://images.novusbio.com/images2/PAK3_AF6897_Immunohistochemistry_11144.jpg)
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![Cyclin B1 Antibody (SPM619) [mFluor Violet 610 SE]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Cyclin B1 Antibody (SPM619) [mFluor Violet 610 SE]")
Secondary Antibodies |
Isotype Controls |
Research Areas for Cyclin B1 Antibody (NBP3-11598MFV610)Find related products by research area.
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| Gene Symbol | CCNB1 |
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